JAC Advance Access originally published online on July 12, 2006
Journal of Antimicrobial Chemotherapy 2006 58(3):511-529; doi:10.1093/jac/dkl277
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© The Author 2006. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org
BSAC standardized disc susceptibility testing method (version 5)
Department of Microbiology, City Hospital NHS Trust Birmingham, B18 7QH, UK
*Corresponding author. Tel: +44-121-507-5693; Fax: +44-121-507-5521; E-mail: jenny.andrews@swbh.nhs.uk
Received 9 May 2006; returned 18 May 2006; revised 31 May 2006; accepted 6 June 2006
Keywords: breakpoints , disc testing , MICs
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Preface |
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The interpretative criteria have been expanded to include recommendations for testing Stenotrophomonas maltophilia, Campylobacter spp.1 and Coryneform organisms and there is limited information for the fast-growing anaerobes Bacteroides fragilis, Bacteroides thetaiotaomicron and Clostridium perfringens. In the section ‘Additional Information’ advice on testing Helicobacter pylori, Brucella and Legionella spp. can be found.1
It is the intention of the British Society for Antimicrobial Chemotherapy (BSAC) and the Journal of Antimicrobial Chemotherapy to publish this document annually. However, as with all methods, it will require constant review and updating. We therefore advise that all interested parties frequently consult the BSAC website (http://www.bsac.org.uk) where the latest updates will be made available.
New or altered text compared with version 42 is indicated in boldface.
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Introduction |
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The BSAC Guide to Sensitivity Testing was first published in 1991 and one of its most important sections was that dealing with
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1 Preparation of plates |
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2 Selection of control organisms |
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3 Preparation of inoculum |
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3.1 Comparison with 0.5 McFarland standard
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3.2 Dilution of suspension adjusted to the turbidity of a 0.5 McFarland standard |
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3.3 Photometric standardization of turbidity of suspensions |
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3.4 Direct susceptibility testing |
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4 Inoculation of agar plates |
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4.2 Use of rotary platers for susceptibility testing
5 Antimicrobial discs
6 Incubation
7 Measuring zones and interpretation
Transparency declarations
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Appendix 1. Testing antimicrobial susceptibility to co-trimoxazole |
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Appendix 2. Efficacy of cefaclor in the treatment of respiratory infections caused by Haemophilus influenzae |
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Cefaclor pharmacokinetics
Cefaclor potency against Haemophilus influenzae
Pharmacodynamics
Conclusion
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Appendix 3 |
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1. Susceptibility testing of Helicobacter pylori
2. Susceptibility testing of Brucella species
3. Susceptibility testing of Legionella species
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