Skip Navigation



JAC Advance Access published online on May 27, 2009
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkp195
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplementary Data
Right arrow All Versions of this Article:
64/2/301    most recent
dkp195v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Rodríguez, I.
Right arrow Articles by Guerra, B.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rodríguez, I.
Right arrow Articles by Guerra, B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2009. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Original research

Extended-spectrum β-lactamases and AmpC β-lactamases in ceftiofur-resistant Salmonella enterica isolates from food and livestock obtained in Germany during 2003–07

Irene Rodríguez1, Wolfgang Barownick2, Reiner Helmuth2, M. Carmen Mendoza1, M. Rosario Rodicio1, Andreas Schroeter2 and Beatriz Guerra2,*

1 Departamento de Biología Funcional, Área de Microbiología, Universidad de Oviedo, 33006 Oviedo, Spain 2 Department of Biological Safety, Federal Institute for Risk Assessment (BfR), Diedersdorfer Weg 1, D-12277 Berlin, Germany

Received 20 February 2009; returned 17 April 2009; revised 28 April 2009; accepted 5 May 2009

* Corresponding author. Tel: +49-30-8412-2082; Fax: +49-30-8412-2953; E-mail: beatriz.guerra{at}bfr.bund.de

Objectives: Detection and characterization of extended-spectrum β-lactamases (ESBLs) and AmpC-encoding genes was conducted in German Salmonella isolated from different sources from 2003 to 2007.

Methods: Non-duplicate German isolates from the National Salmonella Reference Laboratory Collection (2003–07) with ceftiofur MICs of ≥4 mg/L were tested for β-lactam/β-lactamase inhibitor susceptibility, presence of ESBLs or AmpC-encoding genes, class 1 and 2 integrons, other resistance genes, and IS26 and ISEcp1 sequences by PCR/sequencing. The isoelectric point of the β-lactamase was determined. Strains were analysed by PFGE and plasmid profiling. The bla genes were mapped by Southern-blot hybridization. Plasmids were characterized by rep-PCR typing.

Results: Sixteen isolates (10 Salmonella Typhimurium, 2 Salmonella Anatum, 2 Salmonella Paratyphi B dT + , 1 Salmonella Infantis and 1 Salmonella London) carried blaCTX-M (15 blaCTX-M-1 and one blaCTX-M-15) genes located on self-transferable IncB/O, IncI1 and/or IncN plasmids. Seven of the Salmonella Typhimurium isolates carried the SGI1-M variant. Six isolates (five Salmonella Agona and one Salmonella Kentucky) carried the blaCMY-2 gene on IncI1 conjugative plasmids. blaTEM-20 genes were detected in two Salmonella Paratyphi B dT+ isolates, and blaTEM-52 in one Salmonella Paratyphi B dT+ and one Salmonella Virchow, located on IncI1 plasmids. All Salmonella Paratyphi isolates harboured a 2300 bp/dfrA1-sat2-aadA1 class 2 integron.

Conclusions: Among the 22 679 German Salmonella isolates investigated, the ESBL and AmpC β-lactamase prevalence was still low; however, it is slowly increasing. Various β-lactamase genes are linked to a variety of genetic elements capable of horizontal DNA transfer. Consequently, their dissemination is likely and demands adequate risk management strategies.

Key Words: antimicrobial resistance , integron , conjugative plasmid , PFGE , ISEcp1


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Antimicrob ChemotherHome page
J. A. Hammerl, J. Beutlich, S. Hertwig, D. Mevius, E. J. Threlfall, R. Helmuth, and B. Guerra
pSGI15, a small ColE-like qnrB19 plasmid of a Salmonella enterica serovar Typhimurium strain carrying Salmonella genomic island 1 (SGI1)
J. Antimicrob. Chemother., October 27, 2009; (2009) dkp383v1.
[Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.