Skip Navigation



JAC Advance Access published online on January 25, 2008
Journal of Antimicrobial Chemotherapy, doi:10.1093/jac/dkm535
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
61/3/548    most recent
dkm535v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Galani, I.
Right arrow Articles by Giamarellou, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Galani, I.
Right arrow Articles by Giamarellou, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2008. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Evaluation of different laboratory tests for the detection of metallo-β-lactamase production in Enterobacteriaceae

Irene Galani*, Panagiota Danai Rekatsina, Despina Hatzaki, Diamantis Plachouras, Maria Souli and Helen Giamarellou

4th Department of Internal Medicine, Molecular Biology Section, University of Athens Medical School, Athens, Greece

Received 17 November 2007; returned 5 December 2007; revised 28 November 2007; accepted 11 December 2007

* Correspondence address. 4th Department of Internal Medicine, University General Hospital ‘Attikon’, Rimini 1, 124 62 Chaidari, Greece. Tel: +32-105831984; Fax: +32-105326426; E-mail: egalani{at}med.uoa.gr

Objectives: Clinical isolates of Klebsiella pneumoniae (91), Escherichia coli (49), Enterobacter spp. (27), Proteus mirabilis (17), Citrobacter freundii (2), Providencia stuartii (3) and Serratia spp. (5), with various MICs of imipenem, were examined for production of metallo-β-lactamases (MBLs) with different phenotypic laboratory tests that have been previously published to detect MBLs in Pseudomonas aeruginosa and Acinetobacter spp.

Methods: A total of 194 (95 MBL-positive and 99 MBL-negative) clinical isolates with imipenem MICs ≤0.25 to >256 mg/L were examined. All isolates were evaluated by the double-disc synergy test (DDST), the combination disc test (CDT), the MBL Etest and the modified Hodge test. The presence of blaVIM and blaIMP genes was evaluated by in situ hybridization with specific probes and was certified by PCR.

Results: In 30 blaVIM-positive isolates that exhibited MICs of imipenem ≤4 mg/L, MBL Etest could not be evaluated. CDT with ceftazidime and 1900 or 750 µg of EDTA, and DDST after applying an imipenem disc 10 mm apart from a disc containing ~1900 µg of EDTA, showed the highest sensitivity (97.9% to 100%) and specificity (87.9% to 96%) rates among the analysed procedures. CDT with imipenem and 1900 µg of EDTA exhibited a sensitivity of 94.7% and showed very good specificity (98%).

Conclusions: The CDT with imipenem/imipenem+0.5 M EDTA or ceftazidime/ceftazidime+0.2 M EDTA and the DDST with imipenem 10 mm apart from EDTA are the most effective methods for the detection of MBLs in Enterobacteriaceae.

Key Words: MBLs , carbapenemases , EDTA synergy test , Hodge test


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Antimicrob. Agents Chemother.Home page
M. Souli, P. D. Rekatsina, Z. Chryssouli, I. Galani, H. Giamarellou, and K. Kanellakopoulou
Does the Activity of the Combination of Imipenem and Colistin In Vitro Exceed the Problem of Resistance in Metallo-{beta}-Lactamase-Producing Klebsiella pneumoniae Isolates?
Antimicrob. Agents Chemother., May 1, 2009; 53(5): 2133 - 2135.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
T.-t. Qu, J.-l. Zhang, J. Wang, J. Tao, Y.-s. Yu, Y.-g. Chen, J.-y. Zhou, and L.-j. Li
Evaluation of Phenotypic Tests for Detection of Metallo-{beta}-Lactamase-Producing Pseudomonas aeruginosa Strains in China
J. Clin. Microbiol., April 1, 2009; 47(4): 1136 - 1142.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.