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JAC Advance Access originally published online on September 22, 2009
Journal of Antimicrobial Chemotherapy 2009 64(6):1170-1174; doi:10.1093/jac/dkp341
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© The Author 2009. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Original research

Dissemination of transposon Tn6001 in carbapenem-non-susceptible and extensively drug-resistant Pseudomonas aeruginosa in Taiwan

Sung-Pin Tseng1, Jui-Chang Tsai2,3, Lee-Jene Teng1,4,{dagger} and Po-Ren Hsueh4,5,*,{dagger}

1 Department of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University College of Medicine, 1 Chang-Te Street, Taipei 100, Taiwan 2 Center for Optoelectronic Biomedicine, National Taiwan University College of Medicine, 1 Jen-Ai Road, Section 1, Taipei 100, Taiwan 3 Department of Surgery, Division of Neurosurgery, National Taiwan University Hospital, 7 Chung-Shan South Road, Taipei 100, Taiwan 4 Department of Laboratory Medicine, National Taiwan University Hospital, 7 Chung-Shan South Road, Taipei 100, Taiwan 5 Department of Internal Medicine, National Taiwan University Hospital, 7 Chung-Shan South Road, Taipei 100, Taiwan

Received 17 April 2009; returned 15 June 2009; revised 25 August 2009; accepted 25 August 2009

* Corresponding author. Tel: +886-2-23123456, ext. 65355; Fax: +886-2-23224263; E-mail: hsporen{at}ntu.edu.tw

Objectives: To investigate the prevalence of metallo-β-lactamases (MBLs) and Tn6001 in carbapenem-non-susceptible Pseudomonas aeruginosa (CNSPA). The CNSPA included extensively drug-resistant P. aeruginosa (XDRPA) and non-XDRPA isolates in Taiwan.

Methods: A total of 308 CNSPA isolates collected at a medical centre from 2000 to 2005 and 26 XDRPA collected from six medical centres in different regions of Taiwan in 2003 were included. MBL genes and Tn6001 were detected by PCR. Clonal relatedness was determined by PFGE.

Results: Of the 308 CNSPA isolates, 30 (10%) were XDRPA, including 27 (9%) colistin-only-susceptible (COS) and 3 (1%) colistin-only-intermediate (COI) P. aeruginosa. blaVIM-3 was found in 16 (53%) isolates of the XDRPA (n = 30), whereas only 72 (26%) of the non-XDRPA (n = 278) carried the gene. In450 was higher in COS P. aeruginosa (12/27; 44%) than in non-XDRPA isolates (53/278; 19%). Tn6001 was highest in COS P. aeruginosa (11/27; 41%), followed by COI P. aeruginosa (1/3; 33%), and lowest in non-XDRPA (46/278; 17%). Of 26 XDRPA from six medical centres, higher prevalences of blaVIM-3 (16/26; 62%), In450 (16/26; 62%) and Tn6001 (12/26; 46%) were found. Genotyping by PFGE revealed 60 pulsotypes. Hybridization of a blaVIM-3-specific probe following PFGE suggested that the mobile element Tn6001 might have transferred horizontally.

Conclusions: Tn6001 and In450 play an important role in the dissemination of CNSPA and XDRPA. The prevalence of these genetic constituents was higher in XDRPA than in non-XDRPA isolates, suggesting that the mobile element Tn6001 might have transferred horizontally.

Keywords: carbapenem-non-susceptible Pseudomonas aeruginosa (CNSPA) , extensively drug-resistant P. aeruginosa (XDRPA) , metallo-β-lactamases

{dagger} These authors contributed equally to this work.


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