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JAC Advance Access originally published online on August 27, 2009
Journal of Antimicrobial Chemotherapy 2009 64(5):1087-1090; doi:10.1093/jac/dkp310
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© The Author 2009. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Original research

Raltegravir has no residual antiviral activity in vivo against HIV-1 with resistance-associated mutations to this drug

Marc Wirden1,2,*, Anne Simon3, Luminita Schneider2,4, Roland Tubiana2,4, Isabelle Malet1,2, Hocine Ait-Mohand2,4, Gilles Peytavin5, Christine Katlama2,3, Vincent Calvez1,2 and Anne-Genevieve Marcelin1,2

1 Department of Virology, AP-HP, Pitié-Salpêtrière Hospital, Paris, France 2 INSERM U943, Pitié-Salpêtrière Hospital, Paris, France 3 Department of Internal Medicine, AP-HP, Pitié-Salpêtrière Hospital, Paris, France 4 Department of Infectious Diseases, AP-HP, Pitié-Salpêtrière Hospital, Paris, France 5 Department of Pharmacology, AP-HP, Bichat Hospital, Paris, France

Received 22 May 2009; returned 8 July 2009; revised 31 July 2009; accepted 3 August 2009


* Corresponding author. Laboratoire de Virologie, Bât. CERVI, Hôpital Pitié-Salpêtrière, 83, bvd de l'Hopital, 75013 Paris, France. Tel: +33-1-42177401; Fax: +33-1-42177411; E-mail: marc.wirden{at}psl.aphp.fr

Objectives: Emergence of major resistance mutations has already been associated with raltegravir regimen failure. Because of few remaining therapeutic options, the maintenance of raltegravir in the salvage regimen is often considered despite the risk of worsening resistance to integrase inhibitors. We determined whether raltegravir retains residual antiretroviral activity in vivo against viruses harbouring raltegravir mutations, and thus whether the drug can contribute to the subsequent regimen.

Methods: This retrospective observational study reports the changes in the viral load (VL) after the withdrawal of raltegravir from patients carrying virus with resistance mutations. We selected patients under stable treatment and with stable VL during at least the previous 2 months before the withdrawal.

Results: Five patients (A–E) were selected. The median changes in VL and CD4 counts at the end of the raltegravir interruption were –0.04 log copies/mL (range, –0.20 to +0.19) and +58 cells/mm3 (range, –56 to +252), respectively.

Conclusions: All VL changes were well below the clinically relevant variation of 0.5 log copies/mL at the end of the interruption. Thus, this study indicates that, for viruses harbouring one of the two main resistance pathways described for raltegravir, no relevant antiviral activity seems to persist in vivo. Even if further observations would be useful to reinforce this conclusion, the cost/benefit and risk/benefit of maintaining raltegravir as part of a salvage regimen in the presence of raltegravir mutations seem debatable, especially in the absence of relevant antiretroviral activity in this context.

Keywords: N155H , Q148H , residual activity , integrase inhibitor


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