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JAC Advance Access originally published online on May 14, 2009
Journal of Antimicrobial Chemotherapy 2009 64(1):88-93; doi:10.1093/jac/dkp158
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Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Original research

Antimicrobial and antibiofilm efficacy of triclosan and DispersinB® combination

Rabih O. Darouiche1,*, Mohammad D. Mansouri1, Purushottam V. Gawande2 and Srinivasa Madhyastha2

1 Center for Prostheses Infections and Infectious Disease Section, Michael E. Debakey Veterans Affairs Medical Center and Baylor College of Medicine, Houston, TX, USA 2 Kane Biotech Inc., 5-1250 Waverley Street, Winnipeg, MB, Canada R3T 6C6

Received 7 November 2008; returned 21 February 2009; revised 14 March 2009; accepted 9 April 2009

* Corresponding author. Tel: +1-713-799-5088; Fax: +1-713-799-5058; E-mail: rdarouiche{at}aol.com

Objectives: The objectives of this study were to examine: (i) synergy of the combination of triclosan and DispersinB® (DspB); (ii) in vitro efficacy and durability of triclosan + DspB-coated vascular catheters; and (iii) in vivo efficacy of triclosan + DspB-coated catheters compared with chlorhexidine–silver sulfadiazine (CH-SS)-coated and uncoated (control) vascular catheters in preventing colonization by Staphylococcus aureus.

Methods: We investigated the potential synergistic antimicrobial and antibiofilm activity of triclosan and DspB by biofilm assays. The in vitro antimicrobial efficacy of triclosan + DspB-coated catheters was determined by microbial colonization assays. Antimicrobial durability of the coated catheters was tested by soaking segments in bovine serum for 7 days and determining antimicrobial activity, and by a serial plate transfer method. The in vivo efficacy of triclosan + DspB-coated catheters compared with CH-SS-coated and uncoated catheters was assessed by subcutaneous implantation of segments in a rabbit model of S. aureus infection.

Results: The combination of triclosan and DspB showed synergistic antimicrobial and antibiofilm activity against S. aureus, Staphylococcus epidermidis and Escherichia coli, significantly reduced bacterial colonization (P < 0.05) and generally demonstrated a prolonged superior antimicrobial activity against clinical pathogens compared with CH-SS-coated catheters. Triclosan + DspB-coated and CH-SS-coated catheters exhibited equal in vivo efficacy (P ≤ 0.05) in reducing colonization by S. aureus compared with uncoated catheters.

Conclusions: Catheters coated with the triclosan + DspB combination showed synergistic, broad-spectrum and durable antimicrobial activity. Furthermore, the in vivo efficacy of catheters coated with this unique antimicrobial/antibiofilm composition prompts clinical evaluation of such an innovative approach.

Keywords: infection , catheter , bacteria


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