JAC Advance Access originally published online on March 18, 2009
Journal of Antimicrobial Chemotherapy 2009 63(5):895-900; doi:10.1093/jac/dkp068
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Original research |
Novel CTX-M β-lactamase genotype distribution and spread into multiple species of Enterobacteriaceae in Changsha, Southern China
1 Clinical Laboratory, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, Hunan 410008, P. R. China 2 Health Protection Agency West Midlands Public Health Laboratory, Heart of England NHS Foundation Trust, Birmingham B9 5SS, UK 3 School of Immunity and Infection, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK
Received 3 November 2008; returned 16 December 2008; revised 14 February 2009; accepted 15 February 2009
* Corresponding author. Tel/Fax: +86-731-4327437; E-mail: liuwenen{at}hotmail.com
Objectives: The aim of this study was to undertake a survey of the occurrence of CTX-M and SHV extended-spectrum β-lactamase (ESBL) genotypes in Enterobacteriaceae from Hunan Province, China.
Methods: Clinical isolates (425) from three major hospitals in Changsha, Hunan Province, were collected between October 2004 and July 2005, and their antimicrobial susceptibilities of the genotype of blaCTX-M and blaSHV were determined. Random amplified polymorphic DNA was used to characterize the clonality of all of the isolates.
Results: The overall rate of ESBL-positive isolates was 33.4% (142/425). The dominant ESBLs were CTX-M types, and were found in 109/142 (76.8%) isolates comprising seven different genera/species, namely Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae, Enterobacter aerogenes, Citrobacter freundii, Proteus vulgaris and Providencia stuartii. The most common blaCTX-M genotypes were blaCTX-M-14 (47.7%), blaCTX-M-3 (29.4%) and blaCTX-M-15 (17.4%). A novel gene derived from blaCTX-M-15, blaCTX-M-82 (Ala-40
Pro), was identified.
Conclusions: The dominant ESBL genotype in Hunan Province was blaCTX-M. The high prevalence (17.4%) of blaCTX-M-15 has not previously been reported from China. Our results identify that an epidemic of blaCTX-M in Changsha, Hunan Province, has evolved with the appearance and spread of blaCTX-M-15 against the dominant genotypes blaCTX-M-14 and blaCTX-M-3. The worldwide dominance of blaCTX-M-15 could be poised to spread to China, displacing the current prevailing genotypes.
Keywords: ESBLs , blaCTX-M , Hunan Province
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