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JAC Advance Access originally published online on January 18, 2009
Journal of Antimicrobial Chemotherapy 2009 63(3):458-461; doi:10.1093/jac/dkn545
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© The Author 2009. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Original research

Comparison of the in vitro effects of amoxicillin and ampicillin on the polymorphonuclear neutrophil respiratory burst

Marie-Line Reynaert1,2, Anne-Cécile Hochart-Behra2,3,4, Josette Behra-Miellet2,3,*, Bernard Gressier1,4, Louis Mine2, Thierry Dine1, Michel Luyckx1, Luc Dubreuil2 and Claude Brunet1

1 Laboratoire de Pharmacologie, Pharmacocinétique et Pharmacie Clinique, Université Lille 2—Faculté de Pharmacie, 3 rue du Pr Laguesse, BP 83, 59006 Lille Cedex, France 2 Laboratoire de Bactériologie Clinique, Université Lille 2—Faculté de Pharmacie, 3 rue du Pr Laguesse, BP 83, 59006 Lille Cedex, France 3 EA 1043, Université Lille 2—Faculté de Pharmacie, 3 rue du Pr Laguesse, BP 83, 59006 Lille Cedex, France 4 Laboratoire du Centre Hospitalier d'Armentières, 112 rue Sadi Carnot BP 189, 59421 Armentières Cedex, France

Received 2 July 2008; returned 9 September 2008; revised 6 December 2008; accepted 19 December 2008

* Corresponding author. Tel: +33-3-20964040 ext. 4253; Fax: +33-3-20959009; E-mail: josette.behra{at}univ-lille2.fr

Objectives: The aim was to compare the in vitro effects of amoxicillin and ampicillin on the oxidative metabolism of polymorphonuclear neutrophils (PMNs).

Methods: Superoxide radical anion production by PMNs, stimulated or not by various exogenous stimulants and in contact with increasing antibiotic concentrations, was measured using spectrophotometric methods.

Results: Whereas a pro-oxidative action of amoxicillin on PMNs was obtained without exogenous stimulation or with opsonized zymosan (OZ), the O2– production by PMNs incubated with ampicillin did not increase significantly.

Conclusions: This amoxicillin pro-oxidative effect could be due to the activation of the PMN NADPH oxidase, to its induction by a membrane effect or via the OZ pathway. It probably reinforces amoxicillin intrinsic bactericidal action and might partly explain the severe rashes sometimes occurring with amoxicillin treatment.

Keywords: oxidative , superoxide anions , aminopenicillins


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