JAC Advance Access originally published online on October 18, 2008
Journal of Antimicrobial Chemotherapy 2009 63(1):67-71; doi:10.1093/jac/dkn428
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Original research |
Replicon typing of plasmids in Escherichia coli producing extended-spectrum β-lactamases
1 Université Pierre et Marie Curie-Paris-6, Faculté de Médecine, Site Saint-Antoine, Laboratoire de Bactériologie, EA 2392 Paris, France 2 Assistance Publique-Hôpitaux de Paris, Hôpital Tenon, Service de Bactériologie-Hygiène, Paris, France 3 INSERM U722 and Université Denis Diderot-Paris-7, Faculté de Médecine, Site Xavier Bichat, Paris, France 4 INSERM U707, Université Pierre et Marie Curie-Paris-6, Faculté de Médecine, Site Saint-Antoine, Paris, France 5 Assistance Publique-Hôpitaux de Paris, Hôpital Louis Mourier, Laboratoire de Microbiologie, Colombes, France
Received 3 May 2008; returned 23 June 2008; revised 20 September 2008; accepted 22 September 2008
* Correspondence address. Laboratoire de Bactériologie, Hôpital Tenon, 4 rue de la Chine, 75970 Paris Cedex 20, France. Tel: +33-1-56-01-70-18; Fax: +33-1-56-01-61-08; E-mail: guillaume.arlet{at}tnn.aphp.fr
Objectives: Escherichia coli producing CTX-M-15 and CTX-M-14 extended-spectrum β-lactamases (ESBLs) are spreading worldwide. The aim of this work was to investigate the replicons involved in the emergence and spread of ESBLs in relation to ESBL type.
Methods: A collection of 125 TEM, SHV and CTX-M ESBL-producing E. coli strains was analysed. The replicons carrying the ESBLs and the total plasmid content of the strains have been characterized by PCR replicon typing in relation to the type of ESBL. The ESBL replicons were then compared with the replicon content of E. coli strains carrying TEM-1 or inhibitor-resistant TEM (IRT) β-lactamases.
Results: IncF plasmids were the most frequently carried replicons in our collection, but none carried TEM ESBL. Of TEM ESBLs, 67% were carried on IncA/C replicons except for TEM-52 genes, which were carried preferentially on IncI1 replicons. Although CTX-M enzymes can be carried by various replicons, the great majority of genes encoding CTX-M-14 and CTX-M-15 ESBLs were carried by IncF replicons, as were TEM-1 and IRT β-lactamases.
Conclusions: Resistance genes borne by the narrow host-range IncF replicon spread readily as this replicon is well adapted to E. coli. This is observed for blaTEM-1 and blaCTX-M-15 and, to a lesser extent, for blaCTX-M-14. Transposition immunity seems to play an important role in the diffusion process.
Keywords: PCR-based replicon typing , ESBLs , E. coli , transposition immunity
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