JAC Advance Access originally published online on November 11, 2008
Journal of Antimicrobial Chemotherapy 2009 63(1):32-41; doi:10.1093/jac/dkn435
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Original research |
Genetic diversity of methicillin-resistant Staphylococcus aureus carrying type IV SCCmec in Örebro County and the western region of Sweden
1 Department of Clinical Microbiology, Örebro University Hospital, Örebro, Sweden 2 Department of Infection Control Science, Juntendo University, Postgraduate School, Tokyo, Japan 3 Department of Bacteriology, Juntendo University, School of Medicine, Tokyo, Japan 4 Department of Medical Microbiology and Parasitology, China Medical University, Shenyang, China 5 Tuberculosis Research Section, Laboratory of Clinical Infectious Disease, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, USA 6 Department of Infectious Diseases, Örebro University Hospital, Örebro, Sweden
Received 13 June 2008; returned 7 August 2008; revised 30 August 2008; accepted 22 September 2008
* Corresponding author. Present address: Department of Microbiology, Aleris MEDILAB, SE 183 15 Täby, Sweden. E-mail: carolina.berglund{at}aleris.se
Background: Recent studies have shown a predominance of type IV SCCmec among the methicillin-resistant Staphylococcus aureus (MRSA) isolated in the low endemic areas of Örebro County and the western region of Sweden. However, many of these isolates were not possible to classify as existing subtypes IVa, IVb, IVc or IVd.
Methods: We analysed 16 such MRSA isolates by multilocus sequence typing, spa typing, staphylocoagulase (SC) typing and detection of type IVg and IVh SCCmec. MRSA that remained as unknown type IV SCCmec were investigated by long-range PCR covering the J1 region; however, only two isolates were possible to amplify by PCR. The nucleotide sequences of the entire SCCmec of these two MRSA were determined. In addition, isolates that had unknown SC types were investigated by nucleotide sequencing of the coa genes.
Results: Five of 16 isolates were classified as type IVg SCCmec, and four isolates had type IVh SCCmec. Two subtypes of type IV SCCmec shared J1 regions previously identified in other types of SCCmec, types I.2 and II.2. The novel elements were designated as type IVi and IVj SCCmec. In addition, the genetic backgrounds of these Swedish MRSA were diverse and constituted at least nine sequence types and eight SC types, including four new types of SC.
Conclusions: Type IV SCCmec is occurring in heterogeneous clones of MRSA in Sweden, and the majority of the type IV SCCmec were identified in community-acquired MRSA. We describe two novel subtypes of type IV SCCmec with common J1 regions shared by other types of SCCmec, which indicate that J1 regions occurred as primordial SCC.
Keywords: MRSA , community-acquired MRSA , staphylococcal cassette chromosome mec , staphylocoagulase
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  International Working Group on the Classification Classification of Staphylococcal Cassette Chromosome mec (SCCmec): Guidelines for Reporting Novel SCCmec Elements Antimicrob. Agents Chemother., December 1, 2009; 53(12): 4961 - 4967. [Full Text] [PDF]
|
|
|
|
 |
|
 C. Park, H.-H. Shin, E.-Y. Kwon, S.-M. Choi, S.-H. Kim, S. H. Park, J.-H. Choi, J.-H. Yoo, D.-G. Lee, and W. S. Shin Two variants of staphylococcal cassette chromosome mec type IVA in community-associated meticillin-resistant Staphylococcus aureus strains in South Korea J. Med. Microbiol., October 1, 2009; 58(10): 1314 - 1321. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Han, T. Ito, F. Takeuchi, X. X. Ma, M. Takasu, Y. Uehara, D. C. Oliveira, H. de Lencastre, and K. Hiramatsu Identification of a Novel Variant of Staphylococcal Cassette Chromosome mec, Type II.5, and Its Truncated Form by Insertion of Putative Conjugative Transposon Tn6012 Antimicrob. Agents Chemother., June 1, 2009; 53(6): 2616 - 2619. [Abstract] [Full Text] [PDF]
|
|