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JAC Advance Access originally published online on September 28, 2008
Journal of Antimicrobial Chemotherapy 2008 62(6):1277-1280; doi:10.1093/jac/dkn415
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© The Author 2008. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Original research

Rapid method for testing the susceptibility of Aspergillus fumigatus to amphotericin B, itraconazole, voriconazole and posaconazole by assessment of oxygen consumption

Ricardo Araujo1,2,*, Isabel Coutinho1 and Ana Espinel-Ingroff3

1 Department of Microbiology, Faculty of Medicine, University of Porto, Porto, Portugal 2 IPATIMUP, Institute of Pathology and Molecular Immunology, University of Porto, Porto, Portugal 3 Virginia Commonwealth University, Medical Centre, Richmond, VA, USA

Received 25 July 2008; returned 5 August 2008; revised 5 September 2008; accepted 9 September 2008

* Correspondence address. Department of Microbiology, Faculty of Medicine, University of Porto, Porto, Portugal. Tel: +351-916035076; Fax: +351-225513603; E-mail: ricjparaujo{at}yahoo.com

Objectives: Antifungal stress conditions affect fungal germination and growth. The assessment of oxygen consumption resulting from the challenge of Aspergillus fumigatus conidia with antifungal agents might be predictive of the susceptibility of this species to the agents evaluated.

Methods: The antifungal susceptibilities of A. fumigatus to amphotericin B, itraconazole, voriconazole and posaconazole were evaluated for 20 clinical strains by two methods: the rapid assessment of oxygen consumption and the CLSI M38-A2 microdilution method. For the determination of oxygen consumption, conidia were suspended in RPMI 1640 medium with two different concentrations of each antifungal drug (0.25 and 2 mg/L); the oxygen consumption was quantified in a biological oxygen monitor.

Results: A. fumigatus strains showed a wide spectrum of amphotericin B, itraconazole and voriconazole MICs (0.06 to >16 mg/L), but posaconazole MICs ranged from 0.06 to 1 mg/L. Distinct respiratory kinetics, which corresponded to the MIC results, were found. Strains with the highest itraconazole and voriconazole MICs grew faster, undoubtedly consuming the oxygen available in the liquid medium. The reproducibility of this new method was adequate (87%), as well as the agreement with the CLSI method (85%).

Conclusions: Although the potential of this new and rapid method (4–8 versus 48 h CLSI method) for evaluating the susceptibility of A. fumigatus to the antifungal agents has been demonstrated by these preliminary results, further collaborative studies with more isolates should better assess the value of this methodology for testing isolates in the clinical laboratory.

Keywords: Aspergillus spp. , conidial germination , hyphal growth , microdilution method , susceptibility testing


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R. Araujo and A. Espinel-Ingroff
Comparison of Assessment of Oxygen Consumption, Etest, and CLSI M38-A2 Broth Microdilution Methods for Evaluation of the Susceptibility of Aspergillus fumigatus to Posaconazole
Antimicrob. Agents Chemother., November 1, 2009; 53(11): 4921 - 4923.
[Abstract] [Full Text] [PDF]


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