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JAC Advance Access originally published online on September 3, 2008
Journal of Antimicrobial Chemotherapy 2008 62(5):948-950; doi:10.1093/jac/dkn341
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© The Author 2008. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Original research

Plasmid-mediated quinolone resistance in Aeromonas allosaccharophila recovered from a Swiss lake

Renata Cristina Picão1,2, Laurent Poirel1, Antonella Demarta3, Carla Sofia Ferreira Silva1, Anna Rita Corvaglia4, Orlando Petrini3 and Patrice Nordmann1,*

1 Service de Bactériologie-Virologie, INSERM U914 ‘Emerging Resistance to Antibiotics’, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine et Université Paris Sud, K.-Bicêtre, France 2 Laboratório ALERTA, Universidade Federal de São Paulo, São Paulo, Brazil 3 Istituto Cantonale di Microbiologia, Bellinzona, Switzerland 4 Centre Médical et Universitaire, Université de Genève, Geneva, Switzerland

Received 24 June 2008; returned 21 July 2008; revised 23 July 2008; accepted 30 July 2008

* Corresponding author. Tel: +33-1-45-21-36-32; Fax: +33-1-45-21-63-40; E-mail: nordmann.patrice{at}bct.aphp.fr

Objectives: To search for plasmid-mediated qnr genes among waterborne environmental Aeromonas spp. recovered from Switzerland.

Methods: Isolates presenting MICs of nalidixic acid or ciprofloxacin ≥1 mg/L were screened for qnr genes by a multiplex PCR approach followed by sequencing. Plasmids were transferred by transformation, and further analysis of the genetic structures surrounding the qnrS2 gene was carried out by PCR and sequencing.

Results: A qnrS2 gene was identified from a single Aeromonas allosaccharophila isolate (Lugano lake, Lugano), as part of a mobile insertion cassette located on a broad host range IncU-type plasmid. This plasmid co-harboured a class 1 integron containing the aac(6')-Ib-cr, blaOXA-1, catB3 and arr-3 gene cassettes.

Conclusions: These findings strengthen further the role of Aeromonas spp. as a reservoir of antimicrobial resistance determinants in the environment.

Keywords: A. allosaccharophila , QnrS2 , environment


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