Skip Navigation


JAC Advance Access originally published online on April 14, 2008
Journal of Antimicrobial Chemotherapy 2008 62(1):65-71; doi:10.1093/jac/dkn166
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
62/1/65    most recent
dkn166v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Gordon, L.
Right arrow Articles by Giraud, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gordon, L.
Right arrow Articles by Giraud, E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2008. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Original research

Complete sequence of the floR-carrying multiresistance plasmid pAB5S9 from freshwater Aeromonas bestiarum

Laurence Gordon1,*, Axel Cloeckaert2, Benoît Doublet2, Stefan Schwarz3, Agnès Bouju-Albert1, Jean-Pierre Ganière1, Hervé Le Bris1, Anne Le Flèche-Matéos4 and Etienne Giraud1

1 INRA, ENVN, UMR1035 Chimiothérapie Aquacole et Environnement, F-44307 Nantes, France 2 INRA, UR1282 Infectiologie Animale et Santé Publique, F-37380 Nouzilly, France 3 Institut für Nutztiergenetik, Friedrich-Loeffler-Institut (FLI), D-31535 Neustadt-Mariensee, Germany 4 Unité de Biodiversité des Bactéries Pathogènes Emergentes, Institut Pasteur, F-75724 Paris Cedex 15, France

Received 7 January 2008; returned 14 February 2008; revised 11 March 2008; accepted 21 March 2008

* Corresponding author. Tel: +33-2-40-68-78-62; Fax: +33-2-40-68-78-28; E-mail: gordon{at}vet-nantes.fr

Objectives: A multiresistant Aeromonas bestiarum strain, shown to be persistent and spreading in a freshwater stream, was investigated for the presence, location and organization of antimicrobial resistance genes.

Methods: The plasmid pAB5S9 was transferred by electroporation into Escherichia coli TG1. The resistance phenotype mediated by pAB5S9 was determined. Moreover, the plasmid was sequenced completely and analysed for its structure and organization of reading frames.

Results: Plasmid pAB5S9 mediated resistances to phenicols, sulphonamides, streptomycin and tetracycline. The analysis of the 24.7 kb sequence revealed the presence of 20 predicted coding sequences (CDSs), which included the floR, sul2 and strA-strB resistance genes and a tetR-tet(Y) determinant. Approximately 7.5 kb of pAB5S9 showed 100% nucleotide sequence identity to three non-contiguous segments of the SXT element of Vibrio cholerae. Regions identical to SXT comprised the floR gene, flanked upstream by a complete and downstream by a truncated ISCR2 element, and the region of the sul2 and strA-strB genes. Other CDSs of pAB5S9 related to plasmid replication and partitioning, metabolic and gene regulation functions as well as conjugative transfer showed homology to sequences from diverse bacterial species, indicating a mosaic structure.

Conclusions: This study provides the first report of a floR-carrying plasmid in the genus Aeromonas and the first description of a tetR-tet(Y) determinant. The analysis of the multiresistant A. bestiarum strain indicates that strains of this species, some of which are opportunistic pathogens for fish, might also act as a resistance gene reservoir in the freshwater environment.

Keywords: antibiotics , florfenicol resistance , tetracycline resistance , fish-pathogenic bacteria , aquatic environment


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Antimicrob ChemotherHome page
C. Kehrenberg, J. Wallmann, and S. Schwarz
Molecular analysis of florfenicol-resistant Pasteurella multocida isolates in Germany
J. Antimicrob. Chemother., November 1, 2008; 62(5): 951 - 955.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.