JAC Advance Access originally published online on April 10, 2008
Journal of Antimicrobial Chemotherapy 2008 62(1):153-155; doi:10.1093/jac/dkn160
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Original research |
In vitro efficacies of caspofungin or micafungin catheter lock solutions on Candida albicans biofilm growth
Université de Poitiers, UMR CNRS 6008, Equipe de Microbiologie Fondamentale et Appliquée, 6 Rue de la Milètrie, BP 199, 86034 Poitiers Cedex, France
Received 10 December 2007; returned 21 January 2008; revised 7 March 2008; accepted 17 March 2008
* Corresponding author. Tel: +33-5-49-44-37-47; Fax: +33-5-49-44-39-08; E-mail: estelle.cateau{at}chu-poitiers.fr
Objectives: Caspofungin and micafungin belong to the echinocandins; the mechanism of action of echinocandins is based on the inhibition of (1,3)-β-D-glucan synthase. The aim of this study was to investigate in vitro the optimal antifungal lock treatment details against a Candida albicans biofilm.
Methods: An in vitro model of a C. albicans (ATCC 3153 or ATCC 66396) biofilm associated with 100% silicone catheters was used. The effectiveness of the antifungal treatment was assayed against biofilms aged 12 h or 5 days, after exposure to caspofungin (2 mg/L) or micafungin (5 mg/L) for 12 h. The durability of the reduction in the biofilm metabolic activity was investigated (1–3 days after echinocandin treatment). The efficacy of caspofungin and micafungin was determined by evaluating a significant decrease (P < 0.0001) in the metabolic activity of biofilm yeasts.
Results: The results showed that the tested antifungal agents used as lock solution significantly (P < 0.0001) reduced the metabolic activity of C. albicans, whatever the biofilm maturation stage (12 h or 5 days old biofilms). The reduction in the metabolic activity of biofilm yeasts was maintained, even after 48 h.
Conclusions: These data suggest that caspofungin (2 mg/L) and micafungin (5 mg/L) could represent good candidates for the reduction or control of fungal biofilms associated with silicone medical devices, as part of an antifungal lock. They were able to induce a significant and persistent reduction in the yeast metabolic activity of intermediate and mature biofilms, 12 h and 5 days old, respectively, when used as catheter lock solutions.
Keywords: echinocandins , yeasts , silicone , susceptibility
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