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JAC Advance Access originally published online on March 11, 2008
Journal of Antimicrobial Chemotherapy 2008 61(5):1024-1028; doi:10.1093/jac/dkn084
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© The Author 2008. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Original research

The CTX-M-15-producing Escherichia coli diffusing clone belongs to a highly virulent B2 phylogenetic subgroup

Olivier Clermont1,{dagger}, Marie Lavollay2,{dagger}, Sophie Vimont2,3, Catherine Deschamps1,4, Christiane Forestier5, Catherine Branger1,4, Erick Denamur1 and Guillaume Arlet2,3,*

1 INSERM U722 and Université Denis Diderot-Paris 7, Faculté de Médecine, Site Xavier Bichat, Paris, France 2 Université Pierre et Marie Curie-Paris 6, Faculté de Médecine, Laboratoire de Bactériologie, EA 2392 Paris, France 3 Assistance Publique-Hôpitaux de Paris, Hôpital Tenon, Service de Bactériologie-Hygiène, Paris, France 4 Assistance Publique-Hôpitaux de Paris, Hôpital Louis Mourier, Laboratoire de Microbiologie, Colombes, France 5 Université d'Auvergne, Faculté de Pharmacie, Laboratoire de Bactériologie, Clermont-Ferrand, France

Received 16 December 2007; returned 9 January 2008; revised 6 February 2008; accepted 8 February 2008

* Correspondence address. Laboratoire de Bactériologie, Hôpital Tenon, 4 rue de la Chine, 75970 Paris Cedex 20, France. Tel: +33-1-56-01-70-18; Fax: +33-1-56-01-61-08; E-mail: guillaume.arlet{at}tnn.aphp.fr

Objectives: A clone of CTX-M-15-producing Escherichia coli has recently been reported to be spreading through Europe and Africa. The aim of this work was to thoroughly characterize this clone.

Materials and methods: Representative isolates of this clone were subjected to multilocus sequence typing, O typing, virulence gene detection, adhesion assay on human cells, biofilm production assay and mouse lethality assay.

Results: The clone: (i) belongs to a unique B2 phylogenetic subgroup encompassing the pyelonephritogenic diffusely adhering EC7372 strain; (ii) exhibits a specific O25b molecular subtype; (iii) is identical to the E. coli clone O25:H4-ST131 producing CTX-M-15; (iv) produces biofilm; and (v) is highly virulent in mice despite lacking classical extraintestinal pathogenicity islands (except for high pathogenicity island) and the afa/dra gene.

Conclusions: The CTX-M-15-producing E. coli diffusing clone is associated with a high level of antibiotic resistance and with high virulence, showing that, under certain selective pressures, the previously observed trade-off between resistance and virulence may not apply.

Keywords: E. coli , ESBLs , virulence , clonal spread , B2 phylogenetic group

{dagger} O. Clermont and M. Lavollay contributed equally to this work.


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