JAC Advance Access originally published online on January 28, 2008
Journal of Antimicrobial Chemotherapy 2008 61(3):488-497; doi:10.1093/jac/dkm539
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Original research |
Distribution and molecular characterization of tetracycline resistance in Laribacter hongkongensis
1 State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Hong Kong 2 Research Centre of Infection and Immunology, The University of Hong Kong, Hong Kong 3 Department of Microbiology, The University of Hong Kong, Hong Kong
Received 11 October 2007; returned 12 December 2007; revised 3 December 2007; accepted 13 December 2007
* Correspondence address. State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong. Tel: +852-28554892; Fax: +852-28551241; E-mail: kyyuen{at}hkucc.hku.hk
Objectives: Laribacter hongkongensis is a newly discovered bacterium associated with gastroenteritis and found in freshwater fish. Although isolates resistant to tetracycline have been described, their resistance mechanisms have not been studied.
Patients and methods: We describe the distribution and molecular characterization of tetracycline resistance in 48 L. hongkongensis isolates from humans and fish.
Results: Three human isolates and one fish isolate were resistant to tetracycline (MIC 128 mg/L) and doxycycline (MIC 8–16 mg/L) and had reduced susceptibility to minocycline (MIC 1–4 mg/L). A 3566 bp gene cluster, which contains tetR and tetA, was cloned from one of the tetracycline-resistant strains, HLHK5. While the flanking regions and 3' end of the tetA of HLHK5 were identical to the corresponding regions of a tetC island in Chlamydia suis, the tetA gene was almost identical to that of transposon Tn1721 and plasmids of Gram-negative bacteria, suggesting that the tetA/tetR of HLHK5 may have arisen from illegitimate recombination. PCR and DNA sequencing showed the presence of tetA in the other three tetracycline-resistant L. hongkongensis strains. Sequencing and characterization of a 15 665 bp plasmid, pHLHK22, from strain HLHK22 revealed the presence of a similar tetA/tetR gene cluster. This novel plasmid also confers tetracycline resistance when transformed to Escherichia coli and other L. hongkongensis isolates.
Conclusions: Horizontal transfer of genes, especially through Tn1721 and related plasmids, is likely an important mechanism for acquisition and dissemination of tetracycline resistance in L. hongkongensis. The present study is the first report on identification of tetA genes in bacteria of the Neisseriaceae family.
Keywords: freshwater fish , gastroenteritis , tetA gene , tetR gene
These authors contributed equally to this article.