Skip Navigation


JAC Advance Access originally published online on October 3, 2007
Journal of Antimicrobial Chemotherapy 2007 60(6):1370-1374; doi:10.1093/jac/dkm381
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
60/6/1370    most recent
dkm381v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Machado, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Machado, E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2007. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

High diversity of extended-spectrum ß-lactamases among clinical isolates of Enterobacteriaceae from Portugal

Elisabete Machado1,2,3, Teresa M. Coque2, Rafael Cantón2, Ângela Novais2, João Carlos Sousa3, Fernando Baquero2, Luísa Peixe1,* on behalf of The Portuguese Resistance Study Group

1 REQUIMTE, Faculdade de Farmácia, Universidade do Porto, Porto, Portugal 2 Servicio de Microbiología, Hospital Universitario Rámon y Cajal, IMSALUD, Madrid, Spain 3 Faculdade de Ciências da Saúde, Universidade Fernando Pessoa, Porto, Portugal

Received 20 February 2007; returned 8 May 2007; revised 6 September 2007; accepted 10 September 2007

* Correspondence address. Laboratório de Microbiologia, Faculdade de Farmácia da Universidade do Porto, Rua Aníbal Cunha 164, Porto 4050, Portugal Tel: +351-22-200-89-46; Fax: +351-22-200-39-77; E-mail: lpeixe{at}ff.up.pt

Objectives: To investigate the occurrence and the diversity of Ambler class A ESBLs among Enterobacteriaceae from different Portuguese clinical settings over a 2 year period (2002–04).

Methods: One hundred and nine extended-spectrum ß-lactamase (ESBL)-producing Enterobacteriaceae isolates from five geographically distant health institutions in Portugal were studied. ESBLs were characterized by isoelectric focussing, PCR and further sequencing. Antibiotic susceptibility testing, transfer of resistance genes and clonal diversity were determined by standard procedures. Plasmid relatedness was established by comparison of random amplified polymorphic DNA (RAPD) patterns.

Results: ESBLs were identified as TEM (46%), SHV (30%), CTX-M (22%) and GES (2%) types; TEM-24, TEM-52, SHV-12 and CTX-M-15 enzymes being the most frequently found. Inter-hospital dissemination of epidemic strains harbouring the most prevalent ESBLs was detected, including the TEM-24-producing Enterobacter aerogenes European epidemic clone. Conjugative transfer of ESBLs was achieved for 67% of isolates and epidemic plasmids containing specific bla genes were detected (blaCTX-M-15 and blaTEM-24). We describe two new ESBLs, SHV-90 (A187T, G238S and E240K) and SHV-91 (P20S and E240K), and a new TEM-type enzyme conferring a phenotype resembling that of a complex mutant TEM ß-lactamase, designated as TEM-154 (M69L and R164S). The broad-spectrum ß-lactamases SHV-26, SHV-36 and TEM-110 were first observed in our country.

Conclusions: We describe a complex ESBL epidemiology in Portugal, including widespread dissemination of known strains and plasmids coding for TEM-24 and CTX-M-15 enzymes as observed in other European countries.

Keywords: TEM , SHV , CTX-M-15 , clonal dissemination , epidemic plasmids , ESBLs epidemiology


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Antimicrob ChemotherHome page
E. Machado, T. M. Coque, R. Canton, J. C. Sousa, and L. Peixe
Antibiotic resistance integrons and extended-spectrum {beta}-lactamases among Enterobacteriaceae isolates recovered from chickens and swine in Portugal
J. Antimicrob. Chemother., August 1, 2008; 62(2): 296 - 302.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.