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JAC Advance Access originally published online on September 13, 2007
Journal of Antimicrobial Chemotherapy 2007 60(5):1137-1141; doi:10.1093/jac/dkm316
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© The Author 2007. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Characterization of CTX-M and SHV extended-spectrum ß-lactamases and associated resistance genes in Escherichia coli strains of food samples in Tunisia

Ahlem Jouini1,2, Laura Vinué2, Karim Ben Slama1, Yolanda Sáenz2, Naouel Klibi1, Salah Hammami3, Abdellatif Boudabous1 and Carmen Torres2,*

1 Laboratoire MBA, Département de Biologie, Faculté de Sciences de Tunis, Campus Universitaire, 2092 Tunis, Tunisia 2 Area de Bioquímica y Biología Molecular, Universidad de La Rioja, 26006 Logroño, Spain 3 Veterinary Research Institute, Tunis, Tunisia

Received 4 May 2007; returned 26 June 2007; revised 25 July 2007; accepted 31 July 2007

* Corresponding author. Tel: +34-941299750; Fax: +34-941299721; E-mail: carmen.torres{at}unirioja.es

Objectives: To assess the occurrence of extended-spectrum ß-lactamases (ESBLs) in Escherichia coli isolates of faecal samples of animals (n = 40) and food samples (n = 38) obtained in Tunisia in 2006, and to characterize the type of ESBLs, their genetic environments and the associated resistance genes.

Methods: Samples were inoculated in supplemented media (2 mg/L cefotaxime) for isolation of broad-spectrum cephalosporin-resistant E. coli isolates (one isolate/sample). ESBLs and their genetic environments as well as integrons and their gene cassette composition were characterized by PCR and sequencing.

Results: ESBL-producing E. coli isolates were detected in 10 of the 38 food samples analysed (26%) and in none of the tested animal faecal samples. Genes found were as follows (number of isolates): blaCTX-M-1 (5), blaCTX-M-1 + blaTEM-1b (1), blaCTX-M-14 + blaTEM-1b (2), blaCTX-M-8 (1) and blaSHV-5 (1). All ESBL-positive isolates showed unrelated PFGE patterns. ISEcp1 and IS903 were detected surrounding blaCTX-M-14, and ISEcp1/IS26 and orf477 surrounding some of the blaCTX-M-1 genes. Four of the ESBL-positive strains harboured class 1 integrons including different gene cassette combinations.

Conclusions: ESBLs, mainly of the CTX-M class, are detected in E. coli of food origin in Tunisia, being the first time that this mechanism has been detected in food E. coli strains in Africa.

Keywords: CTX-M-1 , CTX-M-8 , CTX-M-14 , SHV-5 , E. coli , animals


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