JAC Advance Access originally published online on June 6, 2007
Journal of Antimicrobial Chemotherapy 2007 60(2):312-316; doi:10.1093/jac/dkm192
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Antifungal susceptibility of Cryptococcus neoformans and Cryptococcus gattii isolates from decayed wood of trunk hollows of Ficus religiosa and Syzygium cumini trees in north-western India
1 Mycology Reference Laboratory, Department of Microbiology, Faculty of Medicine, Kuwait University, Kuwait 2 Department of Medical Mycology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi 110007, India
Received 28 January 2007; returned 17 April 2007; revised 8 April 2007; accepted 4 May 2007
* Corresponding author. Fax: +965-5318454/5332719; E-mail: ziauddin{at}hsc.edu.kw
Background: We present antifungal susceptibility data on environmental isolates of Cryptococcus neoformans (serotype A, n = 117) and Cryptococcus gattii (serotype B, n = 65) cultured from decayed wood of trunk hollows of Ficus religiosa and Syzygium cumini trees.
Methods: Susceptibilities to amphotericin B, fluconazole, ketoconazole, itraconazole and voriconazole were determined by using Etest. The MICs were read after 48 h as per the guidelines provided by the manufacturer.
Results: The MIC90s and susceptibility ranges for C. neoformans isolates were as follows: 0.094 (0.004–0.25) mg/L for amphotericin B, 4 (0.032–12) mg/L for fluconazole, 0.094 (0.004–0.75) mg/L for itraconazole, 0.064 (0.002–0.19) mg/L for ketoconazole, and 0.047 (0.006–0.125) mg/L for voriconazole, whereas for C. gattii isolates these were 0.125 (0.023–0.5) mg/L for amphotericin B, 8 (0.032–16) mg/L for fluconazole, 0.75 (0.006–2) mg/L for itraconazole, 0.125 (0.003–0.19) mg/L for ketoconazole, and 0.094 (0.004–0.125) mg/L for voriconazole. A comparison of the geometric means of MICs (mg/L) revealed that C. gattii was less susceptible than C. neoformans to amphotericin B (0.075 versus 0.051, P = 0.0003), fluconazole (2.912 versus 2.316, P = 0.003), itraconazole (0.198 versus 0.0344, P < 0.0001), ketoconazole (0.072 versus 0.037, P < 0.0001), and voriconazole (0.045 versus 0.023, P < 0.0001).
Conclusions: The antifungal susceptibility data obtained in this study indicate that the occurrence of primary resistance among environmental isolates of C. neoformans serotype A and C. gattii serotype B is rare, and serotype B isolates are less susceptible than serotype A isolates.
Keywords: Etest , fluconazole , itraconazole , voriconazole , amphotericin B
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  C. B. Pereira, F. L. Bueno, A. L. T. Dias, M. R. P. L. Brigagao, C. R. Paula, and A. M. Siqueira Evaluation of laccases and melanization in clinical and environmental Cryptococcus neoformans samples by non-denaturing PAGE J. Med. Microbiol., May 1, 2009; 58(5): 563 - 566. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. R. Thompson III, N. P. Wiederhold, A. W. Fothergill, A. C. Vallor, B. L. Wickes, and T. F. Patterson Antifungal Susceptibilities among Different Serotypes of Cryptococcus gattii and Cryptococcus neoformans Antimicrob. Agents Chemother., January 1, 2009; 53(1): 309 - 311. [Abstract] [Full Text] [PDF]
|
|