Skip Navigation


JAC Advance Access originally published online on April 27, 2007
Journal of Antimicrobial Chemotherapy 2007 59(6):1076-1083; doi:10.1093/jac/dkm095
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
59/6/1076    most recent
dkm095v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (9)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Baixench, M.-T.
Right arrow Articles by Dannaoui, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Baixench, M.-T.
Right arrow Articles by Dannaoui, E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2007. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Acquired resistance to echinocandins in Candida albicans: case report and review

Marie-Thérèse Baixench1, Naji Aoun2, Marie Desnos-Ollivier3, Dea Garcia-Hermoso3, Stéphane Bretagne3, Sandrine Ramires2, Christophe Piketty2 and Eric Dannaoui1,3,*

1 Université Paris Descartes, Faculté de Médecine, AP-HP, Hôpital Européen Georges Pompidou, Unité de Parasitologie—Mycologie, 75015 Paris, France 2 Service d'Immunologie Clinique, Hôpital Européen Georges Pompidou, AP-HP, Paris, France 3 Centre National de Référence Mycologie et Antifongiques, Unité de Mycologie Moléculaire, CNRS URA 3012, Institut Pasteur, 75724 Paris Cedex 15, France

Received 4 January 2007; returned 18 February 2007; revised 7 March 2007; accepted 7 March 2007

* Correspondence address. Centre National de Référence Mycologie et Antifongiques, Unité de Mycologie Moléculaire, CNRS URA3012, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France. Tel: +33-1-40-61-32-50; Fax: +33-1-45-68-84-20; E-mail: dannaoui{at}pasteur.fr

Objectives: A patient with Candida albicans thrush and oesophagitis was treated with high doses of caspofungin but treatment eventually failed. Four C. albicans isolates were serially recovered before and after caspofungin treatment. A microbiological study was performed to characterize these four isolates.

Methods: In vitro antifungal susceptibility testing was performed by the EUCAST reference method in RPMI and AM3 and by Etest®. Molecular typing of the four isolates was done by sizing three polymorphic microsatellite markers. To look for specific mutations, sequencing of a region of the gene encoding the 1-3-ß-D-glucan synthase was performed for the four isolates.

Results: In vitro antifungal susceptibility testing showed an increase in both caspofungin and micafungin MICs for the two isolates recovered after caspofungin treatment failure. The best discrimination between the pre-treatment and post-treatment isolates was obtained with Etest®. Molecular typing of the four isolates showed that the post-treatment isolates with reduced susceptibility were identical to a susceptible pre-treatment isolate, suggesting the acquisition of caspofungin resistance. Sequencing of the gene encoding the 1-3-ß-D-glucan synthase showed a mutation responsible for an amino acid change at Phe-641 that could confer reduced susceptibility to both echinocandins.

Conclusions: Our results indicate that is it useful to perform in vitro susceptibility testing in the cases of clinical failure during caspofungin therapy.

Keywords: caspofungin , micafungin , 1-3-ß-D-glucan synthase , Etest


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Clin. Microbiol.Home page
M. A. Pfaller, D. J. Diekema, L. Ostrosky-Zeichner, J. H. Rex, B. D. Alexander, D. Andes, S. D. Brown, V. Chaturvedi, M. A. Ghannoum, C. C. Knapp, et al.
Correlation of MIC with Outcome for Candida Species Tested against Caspofungin, Anidulafungin, and Micafungin: Analysis and Proposal for Interpretive MIC Breakpoints
J. Clin. Microbiol., August 1, 2008; 46(8): 2620 - 2629.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
M. Desnos-Ollivier, F. Dromer, and E. Dannaoui
Detection of Caspofungin Resistance in Candida spp. by Etest
J. Clin. Microbiol., July 1, 2008; 46(7): 2389 - 2392.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
M. A. Pfaller, V. Chaturvedi, D. J. Diekema, M. A. Ghannoum, N. M. Holliday, S. B. Killian, C. C. Knapp, S. A. Messer, A. Miskov, and R. Ramani
Clinical Evaluation of the Sensititre YeastOne Colorimetric Antifungal Panel for Antifungal Susceptibility Testing of the Echinocandins Anidulafungin, Caspofungin, and Micafungin
J. Clin. Microbiol., July 1, 2008; 46(7): 2155 - 2159.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
J. D. Cleary, G. Garcia-Effron, S. W. Chapman, and D. S. Perlin
Reduced Candida glabrata Susceptibility Secondary to an FKS1 Mutation Developed during Candidemia Treatment
Antimicrob. Agents Chemother., June 1, 2008; 52(6): 2263 - 2265.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
S. Hodgetts, L. Nooney, R. Al-Akeel, A. Curry, S. Awad, R. Matthews, and J. Burnie
Efungumab and caspofungin: pre-clinical data supporting synergy
J. Antimicrob. Chemother., May 1, 2008; 61(5): 1132 - 1139.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
E. Dannaoui, O. Lortholary, D. Raoux, M. E. Bougnoux, G. Galeazzi, C. Lawrence, D. Moissenet, I. Poilane, D. Hoinard, F. Dromer, et al.
Comparative In Vitro Activities of Caspofungin and Micafungin, Determined Using the Method of the European Committee on Antimicrobial Susceptibility Testing, against Yeast Isolates Obtained in France in 2005-2006
Antimicrob. Agents Chemother., February 1, 2008; 52(2): 778 - 781.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
R. D. Cannon, E. Lamping, A. R. Holmes, K. Niimi, K. Tanabe, M. Niimi, and B. C. Monk
Candida albicans drug resistance another way to cope with stress
Microbiology, October 1, 2007; 153(10): 3211 - 3217.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.