Skip Navigation


JAC Advance Access originally published online on March 15, 2007
Journal of Antimicrobial Chemotherapy 2007 59(5):1001-1004; doi:10.1093/jac/dkm058
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
59/5/1001    most recent
dkm058v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (19)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Ruzin, A.
Right arrow Articles by Bradford, P. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ruzin, A.
Right arrow Articles by Bradford, P. A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2007. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

AdeABC multidrug efflux pump is associated with decreased susceptibility to tigecycline in Acinetobacter calcoaceticus–Acinetobacter baumannii complex

Alexey Ruzin*, David Keeney and Patricia A. Bradford

Department of Infectious Disease, Wyeth Research, Pearl River, NY 10965, USA

Received 25 October 2006; returned 5 December 2006; revised 21 December 2006; accepted 5 February 2007

* Corresponding author. Tel: +1-845-602-4592; Fax: +1-845-602-5671; E-mail: ruzina{at}wyeth.com

Objectives: To investigate the role of the AdeABC multidrug efflux pump in the decreased susceptibility of clinical isolates of Acinetobacter calcoaceticus–Acinetobacter baumannii complex to tigecycline.

Methods: Gene expression was analysed by Taqman RT-PCR. A single cross-over achieved insertional inactivation of the adeB gene with a suicide plasmid construct carrying an adeB fragment obtained by PCR. Analysis of the adeRS locus was performed by PCR and sequencing. Ribotyping was performed with the RiboPrinter system. MICs were determined by Etest.

Results: Expression analysis revealed constitutive overexpression of adeABC in less-susceptible clinical isolates G5139 and G5140 (tigecycline MIC = 4 mg/L) when compared with the isogenic clinical isolates G4904 and G5141 (MIC = 1.5 mg/L). Insertional mutants GC7945 (adeB knockout in G5139) and GC7951 (adeB knockout in G5140) were obtained, which resulted in tigecycline MICs of 0.5 mg/L. As reported previously, the expression of adeABC is regulated by the two-component signalling system encoded by the adeR and adeS genes. PCR and sequencing analyses revealed an insertion of an ISABA-1 element in the adeS gene of G5139 and G5140.

Conclusions: The results of this study suggest that decreased susceptibility to tigecycline in the A. calcoaceticus–A. baumannii complex is associated with the overexpression of the AdeABC multidrug efflux pump.

Keywords: Acinetobacter , antibiotic resistance , efflux pump expression , RT-PCR , gene inactivation


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Antimicrob ChemotherHome page
D. C. Bean and D. W. Wareham
Paradoxical effect of 1-(1-naphthylmethyl)-piperazine on resistance to tetracyclines in multidrug-resistant Acinetobacter baumannii
J. Antimicrob. Chemother., February 1, 2009; 63(2): 349 - 352.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
S. Bratu, D. Landman, D. A. Martin, C. Georgescu, and J. Quale
Correlation of Antimicrobial Resistance with {beta}-Lactamases, the OmpA-Like Porin, and Efflux Pumps in Clinical Isolates of Acinetobacter baumannii Endemic to New York City
Antimicrob. Agents Chemother., September 1, 2008; 52(9): 2999 - 3005.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
A. Ruzin, F. W. Immermann, and P. A. Bradford
Real-Time PCR and Statistical Analyses of acrAB and ramA Expression in Clinical Isolates of Klebsiella pneumoniae
Antimicrob. Agents Chemother., September 1, 2008; 52(9): 3430 - 3432.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
P. M. Hawkey
The growing burden of antimicrobial resistance
J. Antimicrob. Chemother., September 1, 2008; 62(suppl_1): i1 - i9.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
A. Y. Peleg, H. Seifert, and D. L. Paterson
Acinetobacter baumannii: Emergence of a Successful Pathogen
Clin. Microbiol. Rev., July 1, 2008; 21(3): 538 - 582.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
S. Begic and E. A. Worobec
The role of the Serratia marcescens SdeAB multidrug efflux pump and TolC homologue in fluoroquinolone resistance studied via gene-knockout mutagenesis
Microbiology, February 1, 2008; 154(2): 454 - 461.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
L. Damier-Piolle, S. Magnet, S. Bremont, T. Lambert, and P. Courvalin
AdeIJK, a Resistance-Nodulation-Cell Division Pump Effluxing Multiple Antibiotics in Acinetobacter baumannii
Antimicrob. Agents Chemother., February 1, 2008; 52(2): 557 - 562.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
R. R. Reinert, D. E. Low, F. Rossi, X. Zhang, C. Wattal, and M. J. Dowzicky
Antimicrobial susceptibility among organisms from the Asia/Pacific Rim, Europe and Latin and North America collected as part of TEST and the in vitro activity of tigecycline
J. Antimicrob. Chemother., November 1, 2007; 60(5): 1018 - 1029.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
F. Perez, A. M. Hujer, K. M. Hujer, B. K. Decker, P. N. Rather, and R. A. Bonomo
Global Challenge of Multidrug-Resistant Acinetobacter baumannii
Antimicrob. Agents Chemother., October 1, 2007; 51(10): 3471 - 3484.
[Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
A. Nemec, M. Maixnerova, T. J. K. van der Reijden, P. J. van den Broek, and L. Dijkshoorn
Relationship between the AdeABC efflux system gene content, netilmicin susceptibility and multidrug resistance in a genotypically diverse collection of Acinetobacter baumannii strains
J. Antimicrob. Chemother., September 1, 2007; 60(3): 483 - 489.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
D. Curcio and F. Fernandez
Acinetobacter spp. susceptibility to tigecycline: a worldwide perspective
J. Antimicrob. Chemother., August 1, 2007; 60(2): 449 - 450.
[Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.