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JAC Advance Access originally published online on March 9, 2007
Journal of Antimicrobial Chemotherapy 2007 59(4):742-745; doi:10.1093/jac/dkl538
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© The Author 2007. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Characterization of sulphonamide resistance genes and class 1 integron gene cassettes in Enterobacteriaceae, Central African Republic (CAR)

Thierry Frank1,2, Valérie Gautier2, Antoine Talarmin1, Raymond Bercion1 and Guillaume Arlet2,3,*

1 Institut Pasteur de Bangui, Bangui, Central African Republic 2 Université Pierre et Marie Curie, Paris 6, EA 2392, Laboratoire de Bactériologie, F-75005, Paris, France 3 Service de Bactériologie-Hygiène, Hôpital Tenon, AP-HP, Paris, France

Received 11 July 2006; returned 8 August 2006; revised 11 December 2006; accepted 12 December 2006

* Corresponding author. Service de Bactériologie-Hygiène, Hôpital Tenon, AP-HP, rue de la Chine, 75970 Paris cedex 20, France. Tel: +33 1 56 01 70 1; Fax: +33 1 56 01 61 08; E-mail: guillaume.arlet{at}tnn.aphp.fr

Objectives: The aim of this study was to characterize genes encoding sulphonamide resistance and gene cassettes associated with class 1 integrons in trimethoprim-sulphamethoxazole resistant Enterobacteriaceae recovered from Bangui, Central African Republic (CAR).

Methods: We studied 78 clinical Enterobacteriaceae isolates, including 16 extended-spectrum ß-lactamases producers, 10 Salmonella and 9 Shigella, resistant to trimethoprim-sulphamethoxazole as assessed by the disc diffusion method. PCR was used to test for sul1 and sul2 genes. Class 1 integron resistance gene cassettes were characterized by directly sequencing PCR products obtained with primers recognising 5' and 3' conserved regions.

Results: The sul1 gene was found in 67 isolates, the sul2 gene in 72 isolates and both genes in 62 isolates, while the int1 gene was found in 74 isolates. The most prevalent dfr genes were dfrA7 (49%), dfrA1 (17%) and dfrA2d (13%).

Conclusion: These results illustrate the wide distribution of sulphonamide and trimethoprim resistance genes among Enterobacteriaceae in Bangui (CAR).

Keywords: co-trimoxazole resistance, gene cassettes, antimicrobial agents


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