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JAC Advance Access originally published online on February 8, 2007
Journal of Antimicrobial Chemotherapy 2007 59(3):465-472; doi:10.1093/jac/dkl535
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© The Author 2007. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Genetic relatedness between methicillin-susceptible and methicillin-resistant Staphylococcus aureus: results of a national survey

M. Hallin*, O. Denis, A. Deplano, R. De Mendonça, R. De Ryck, S. Rottiers and M. J. Struelens

Laboratoire de Référence MRSA-Staphylocoques, Service de Microbiologie, Université Libre de Bruxelles, Hôpital Erasme, 1070 Brussels, Belgium

Received 3 October 2006; returned 26 October 2006; revised 11 December 2006; accepted 11 December 2006

* Corresponding author. Tel: +32-2-555-34-84; Fax: +32-2-555-31-10; E-mail: mahallin{at}ulb.ac.be

Objectives: Surveillance of hospital-acquired methicillin-resistant Staphylococcus aureus (HA-MRSA) infections has shown the emergence and spread of several epidemic MRSA clones over the past 10 years in Belgium. Whether these clones have been imported from abroad or else have arisen locally via staphylococcal cassette chromosome mec (SCCmec) acquisition by successful methicillin-susceptible S. aureus (MSSA) clones is unknown.

Methods: We determined by PFGE, spa typing, multi-locus sequence typing (MLST) and agr group analysis the genetic relatedness of 103 MSSA and 511 MRSA strains from a national survey of patients admitted to 112 Belgian hospitals in 2003.

Results: The 103 MSSA strains presented very diverse genetic backgrounds, they were distributed into 40 distinct PFGE types and clustered in 15 distinct MLST CCs. Up to 45% harboured the same genotype as five major epidemic HA-MRSA clones. These MRSA clones all harbour a type IV SCCmec element.

Conclusions: These findings are consistent with multiple recent acquisitions of the more mobile type IV SCCmec by MSSA and suggest that certain genetic backgrounds are conferring a selective advantage, regardless of the resistance profile. However, since the predominant MSSA and MRSA lineages identified in Belgium are disseminated worldwide, importation of epidemic MRSA strains remains an alternative hypothesis.

Keywords: epidemiology , evolution , pulsed field gel electrophoresis , Belgium


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