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JAC Advance Access originally published online on February 8, 2007
Journal of Antimicrobial Chemotherapy 2007 59(3):403-410; doi:10.1093/jac/dkl491
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© The Author 2007. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Antibiotic resistance and molecular epidemiology of Escherichia coli O26, O103 and O145 shed by two cohorts of Scottish beef cattle

Leila Vali1, Ahmed Hamouda1, Deborah V. Hoyle1, Michael C. Pearce2, Lucy H. R. Whitaker1, Claire Jenkins3, Hazel I. Knight2, Alastair W. Smith2 and Sebastian G. B. Amyes1,*

1 Molecular Chemotherapy, Centre for Infectious Diseases, The Chancellor's Building, 49 Little France Crescent, University of Edinburgh, Edinburgh EH16 4SB, UK 2 Scottish Agricultural College, Drummondhill, Stratherrick Road, Inverness IV2 4JZ, UK 3 Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Imperial College London, London SW7 2AZ, UK

Received 10 August 2006; returned 14 September 2006; revised 2 November 2006; accepted 9 November 2006

* Corresponding author. Tel: +44-131-2426461; Fax: +44-131-2429375; E-mail: s.g.b.amyes{at}ed.ac.uk

Objectives: The aim of this study was to identify the profile of antibiotic resistance among E. coli O26, O103 and O145 in two cohorts of Scottish beef cattle on two farms and to determine whether there is an association between resistant phenotypes and the genotypic PFGE patterns to suggest clonality among resistant strains.

Methods: MICs of 11 antibiotics for 297 E. coli O26, 152 E. coli O103 and 13 E. coli O145 were determined. Isolates were screened for the presence integrons 1 and 2 and the virulence factors stx1, stx2, eaeA and ehxA by PCR with specific primers. PFGE subtyping was performed after digestion with XbaI endonuclease.

Results: Among E. coli O26, O103 and O145 there were four, four and one isolates, respectively, that harboured a class 1 integron. A class 2 integron was detected in only one O145 isolate. Diversity in PFGE patterns was higher among E. coli O103 and O145 strains compared with the O26 serotype; and PFGE demonstrated 13, 27 and 6 different patterns among O26, O103 and O145 isolates, respectively. Selective PFGE types that harboured virulence factors were widespread among the cattle population throughout the sampling period. There were multiply resistant isolates that were of similar PFGE patterns.

Conclusions: The dissemination and persistence of certain PFGE genotypes among the cattle population was evident in this study. Certain resistance phenotypes, especially among E. coli O26 isolates, were associated with distinct PFGE clones.

Keywords: Escherichia coli O26 , E. coli O103 , E. coli O145 , PFGE , MIC , Stx , eaeA


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