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JAC Advance Access originally published online on October 9, 2006
Journal of Antimicrobial Chemotherapy 2006 58(6):1250-1253; doi:10.1093/jac/dkl407
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© The Author 2006. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Expression of the las and rhl quorum-sensing systems in clinical isolates of Pseudomonas aeruginosa does not correlate with efflux pump expression or antimicrobial resistance

Simona Bratu, Jyoti Gupta and John Quale*

Division of Infectious Diseases, State University of New York Downstate Medical Center 450 Clarkson Avenue, Brooklyn, NY 11203, USA

Received 30 June 2006; returned 17 August 2006; revised 6 September 2006; accepted 12 September 2006

*Corresponding author: Tel: +1-718-270-2148; Fax: +1-718-270-2465; E-mail: jquale{at}downstate.edu

Objectives: Quorum-sensing systems regulate expression of several virulence factors and may affect the MexAB-OprM efflux system in Pseudomonas aeruginosa. This study investigated the relationship between two quorum-sensing systems, efflux pump MexAB-OprM expression and antimicrobial resistance in 33 clinical isolates of P. aeruginosa.

Methods: Expression of the quorum-sensing regulatory genes lasR and rhlR was assessed by real time RT–PCR. The autoinducer synthetase genes lasI and rhlI and the regulatory genes mexT and mexS were characterized by DNA sequencing. Production of pyocyanin and elastase in each of the isolates was also determined.

Results: While there was a significant correlation between expression of the quorum-sensing regulatory genes and production of pyocyanin and elastase, there was no correlation with expression of mexA or with antimicrobial resistance. There were no mutations in lasI, rhlI, mexT or mexS that correlated with quorum-sensing expression.

Conclusions: Increased activity of two quorum-sensing systems in P. aeruginosa does not contribute to increased mexA expression or antimicrobial resistance.

Keywords: virulence mechanisms , resistance mechanisms , ß-lactamase


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