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JAC Advance Access originally published online on October 25, 2006
Journal of Antimicrobial Chemotherapy 2006 58(6):1133-1138; doi:10.1093/jac/dkl423
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© The Author 2006. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Diverse class 2 integrons in bacteria from beef cattle sources

Robert S. Barlow* and Kari S. Gobius

Food Science Australia, PO Box 3312 Tingalpa DC, Queensland 4173, Australia

Received 24 July 2006; returned 12 September 2006; revised 20 September 2006; accepted 25 September 2006

*Correspondence address. Food Science Australia, Cnr Wynnum and Creek Roads, Cannon Hill, QLD 4170, Australia. Tel: +61-7-3214-2035; Fax: +61-7-3214-2062; E-mail: Robert.Barlow{at}csiro.au

Objectives: The purpose of this study was to determine the diversity of class 2 integrons in bacteria isolated from beef cattle sources.

Methods: The variable regions of a subset of 11 class 2 integron-containing bacteria were analysed by PCR and DNA sequencing for the presence of novel rearrangements.

Results: A total of six different class 2 integron arrays were identified and four of these were fully characterized. Three of the four arrays characterized have been previously described; however the remaining array is unlike previously described class 2 integrons. The novel class 2 integron was found in Providencia stuartii and contains an apparently functional class 2 integrase. Examination of the variable region of the P. stuartii integron identified nine open reading frames, mostly of unknown function, and represents the first report of a class 2 integron without inserted antibiotic resistance gene cassettes.

Conclusions: This study has identified a novel class 2 integron found in P. stuartii that contains an apparently functional naturally occurring class 2 integrase. Further investigation of this novel class 2 integron is required to determine the impact of a functional class 2 integrase upon the evolution of class 2 integrons.

Keywords: Providencia , functional integrase , integron evolution


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