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JAC Advance Access originally published online on October 20, 2006
Journal of Antimicrobial Chemotherapy 2006 58(6):1124-1132; doi:10.1093/jac/dkl400
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© The Author 2006. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Class 1 and class 2 integrons in non-prevalent serovars of Salmonella enterica: structure and association with transposons and plasmids

Irene Rodríguez1, M. Cruz Martín2, M. Carmen Mendoza1 and M. Rosario Rodicio1,*

1 Departamento de Biología Funcional-Microbiología, Universidad de Oviedo and Instituto Universitario de Biotecnología de Asturias (IUBA) 33006-Oviedo, Spain 2 Instituto de Productos Lácteos de Asturias (CSIC) 33300-Villaviciosa, Asturias, Spain

Received 26 July 2006; returned 30 August 2006; revised 7 September 2006; accepted 11 September 2006

*Correspondence address. Área de Microbiología, Facultad de Medicina, Universidad de Oviedo, Julián Clavería 6, 33006-Oviedo, Spain. Tel: +34-985103562; Fax: +34-985103148; E-mail: rrodicio{at}fq.uniovi.es

Objectives: To characterize class 1 and class 2 integrons which were simultaneously detected in non-typhoid Salmonella enterica strains of non-prevalent serovars, and to investigate their possible association with transposons and/or plasmids.

Methods: Eight multidrug-resistant S. enterica strains belonging to serovars Virchow (4), Panama (2), Grumpensis (1) and Worthington (1), each containing a class 1 and a class 2 integron, were analysed. Nested PCR amplification was used to determine the gene-cassette configuration of the integrons. Overlapping PCR amplifications were applied in integron–transposon linkage experiments. Conjugation and hybridization experiments were used to localize integrons and transposons in the bacterial genome (plasmid and chromosome associated).

Results: One of two different class 1 integrons (with variable regions of 1000 bp/aadA1 and 2300 bp/sat-smr-aadA1) inserted into Tn21-like transposons, were found to coexist with the class 2 integron (2300 bp/dfrA1-sat1-aadA1) of Tn7 in the analysed strains. Class 1 integrons were always found in large conjugative plasmids whereas apparently intact or defective copies of the Tn7 integron could be located on the same plasmid and/or the bacterial chromosome.

Conclusions: This report describes different associations between mobile genetic elements that play a crucial role in the capture and spread of antimicrobial drug resistance. As far as we are aware, this is the first description of class 2 integrons in serovars Panama, Grumpensis and Worthington.

Keywords: multidrug resistance , mobile genetic elements , gene mapping , PFGE


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N. Martinez, M. C. Mendoza, I. Rodriguez, S. Soto, M. Bances, and M. R. Rodicio
Detailed structure of integrons and transposons carried by large conjugative plasmids responsible for multidrug resistance in diverse genomic types of Salmonella enterica serovar Brandenburg
J. Antimicrob. Chemother., December 1, 2007; 60(6): 1227 - 1234.
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