Skip Navigation


JAC Advance Access originally published online on September 14, 2006
Journal of Antimicrobial Chemotherapy 2006 58(5):942-949; doi:10.1093/jac/dkl377
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
58/5/942    most recent
dkl377v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (3)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Ishida, K.
Right arrow Articles by Nakamura, C. V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ishida, K.
Right arrow Articles by Nakamura, C. V.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2006. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Influence of tannins from Stryphnodendron adstringens on growth and virulence factors of Candida albicans

Kelly Ishida1, João Carlos Palazzo de Mello2, Diógenes Aparício Garcia Cortez2, Benedito Prado Dias Filho3, Tânia Ueda-Nakamura3 and Celso Vataru Nakamura3,*

1 Mestranda do Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Estadual de Maringá Avenida Colombo, 5790, 87020-900, Maringá, Paraná, Brazil 2 Departamento de Farmácia e Farmacologia, Universidade Estadual de Maringá Avenida Colombo, 5790, 87020-900, Maringá, Paraná, Brazil 3 Departamento de Análises Clínicas, Universidade Estadual de Maringá Avenida Colombo, 5790, 87020-900, Maringá, Paraná, Brazil

Received 29 May 2006; returned 23 June 2006; revised 10 August 2006; accepted 22 August 2006

*Corresponding author. Tel: +55-44-3261-4863; Fax: +55-44-3261-4860; E-mail: cvnakamura{at}uem.br

Objectives: The main objective of this work was to investigate the antifungal activity of a crude extract, fractions and subfractions from Stryphnodendron adstringens (Mart.) Coville, known as ‘barbatimão’.

Methods: The growth inhibition by ‘barbatimão’ of 103 isolates of yeasts from vaginal fluid was determined using the broth microdilution method. In addition, the effect of the most active subfraction on cell surface hydrophobicity (CSH), germ-tube formation, budding, ultrastructure and phagocytosis of Candida albicans was analysed. Fluconazole and nystatin were used as reference drugs. The cytotoxicity of ‘barbatimão’ to Vero cells, macrophages and red blood cells was assessed. The most active subfraction was characterized by mass and 13C NMR spectroscopy.

Results: Subfraction F2.4 had the best antifungal action at concentrations above 7.80 mg/L. Its action was similar to nystatin, and only slightly less effective than fluconazole. CSH and the capacity for adhering to Vero cells and a glass surface were lower in treated yeasts. In addition, the inhibition of formation of the germ tube, the increase in the number of buds and changes in the cell wall ultrastructure of C. albicans were also demonstrated. ‘Barbatimão’ extracts showed low cytotoxicity to Vero cells, macrophages and red blood cells. Subfraction F2.4 is composed of proanthocyanidin polymers of prodelphinidin and prorobinetinidin units and gallic acid of molecular weight 2114 Da.

Conclusions: The antifungal action of subfraction F2.4 on C. albicans can be attributed to condensed tannins. It is considered moderate antifungal activity. These properties of ‘barbatimão’ on the growth of C. albicans, putative virulence factors and its low cytotoxicity justify further studies to investigate the mechanisms of action and the possible development of a new antifungal agent.

Keywords: antifungal , yeasts , proanthocyanidin , electron microscopy , hydrophobicity


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.