Skip Navigation


JAC Advance Access originally published online on September 15, 2006
Journal of Antimicrobial Chemotherapy 2006 58(5):1044-1047; doi:10.1093/jac/dkl366
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
58/5/1044    most recent
dkl366v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (2)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Agersø, Y.
Right arrow Articles by Aarestrup, F. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Agersø, Y.
Right arrow Articles by Aarestrup, F. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2006. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

dfrA25, a novel trimethoprim resistance gene from Salmonella Agona isolated from a human urine sample in Brazil

Yvonne Agersø1,*, Gisele Peirano2 and Frank M. Aarestrup1

1 WHO Collaborating Centre for Antimicrobial Resistance in Foodborne Pathogens, Danish Institute for Food and Veterinary Research Bulowsvej 27, DK-1790 Copenhagen V, Denmark 2 Oswaldo Cruz Institute, Avenida Brasil 4365 21045-900 Rio de Janeiro, Brazil

Received 28 May 2006; returned 19 July 2006; revised 14 August 2006; accepted 14 August 2006

*Corresponding author. Tel: +45-72-34-6000; Fax: +45-72-34-6001; E-mail: ya{at}dfvf.dk

Objectives: To describe a novel trimethoprim resistance gene, designated dfrA25, which was detected as a gene cassette within a class 1 integron in Salmonella Agona.

Methods: The gene was cloned into Escherichia coli MT102 and resistance to 10 different antimicrobial drugs was measured. A phylogenetic tree was constructed based on representative trimethoprim-resistance-mediating DfrA proteins retrieved from GenBank. Filter-mating experiments and Southern blots of plasmid preparations were performed with the donor and selected transconjugants.

Results and conclusions: dfrA25 encodes a dihydrofolate reductase of 157 amino acids with closest identity (85%) to dfrA5 dihydrofolate reductase. dfrA25 was located on a transferable plasmid (~150 kb) that also harboured the tetracycline resistance gene tet(A).

Keywords: integrons , gene cassettes , dihydrofolate reductase


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Antimicrob ChemotherHome page
Q. Wei, X. Jiang, Z. Yang, N. Chen, X. Chen, G. Li, and Y. Lu
dfrA27, a new integron-associated trimethoprim resistance gene from Escherichia coli
J. Antimicrob. Chemother., February 1, 2009; 63(2): 405 - 406.
[Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.