Skip Navigation


JAC Advance Access originally published online on June 27, 2006
Journal of Antimicrobial Chemotherapy 2006 58(3):661-664; doi:10.1093/jac/dkl267
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
58/3/661    most recent
dkl267v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by de la Prieta, M. C.
Right arrow Articles by Lobo, J. M. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by de la Prieta, M. C.
Right arrow Articles by Lobo, J. M. G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2006. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Characterization of defective ß-lactamase genes in Yersinia enterocolitica

María Carmen de la Prieta1, María Victoria Francia2, Asunción Seoane1 and Juan M. García Lobo1,*

1 Departamento de Biología Molecular, Facultad de Medicina, Universidad de Cantabria Santander, Spain 2 Servicio de Microbiología, Hospital Universitario Marqués de Valdecilla Santander, Spain

Received 7 April 2006; returned 3 May 2006; revised 8 May 2006; accepted 1 June 2006

*Corresponding author. Tel: +34-942-201948; Fax +34-942-201945; E-mail: jmglobo{at}unican.es

Objectives: To study at the molecular level the heterogeneity of expression of the two chromosomal ß-lactamases, BlaA and BlaB, in Yersinia enterocolitica strains isolated from clinical samples.

Methods: MIC determination by the agar dilution method and ß-lactamase assays was performed to determine the resistance level conferred by these enzymes. DNA cloning, PCR and direct sequencing were used to detect the presence of mutations.

Results: The blaA allele from strain IP97 (blaA97) was found to carry a deletion of 51 bp which entirely abolished its ß-lactamase activity. Both the ampR gene and the promoter region of strain Y56 were shown to be functional by a gene swapping experiment. The blaB allele from strain Y56 was found to carry two point mutations, only one of them resulting in a change in the amino acid sequence of the protein. This single amino acid change created a practically inactive BlaB or AmpC cephalosporinase in Y. enterocolitica Y56.

Conclusions: The lack of activity observed in the ß-lactamases of some Y. enterocolitica isolates was due to the presence of point mutations or small deletions in the corresponding genes.

Keywords: Y. enterocolitica , resistance mechanisms , ß-lactams , molecular characterization


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.