JAC Advance Access originally published online on May 30, 2006
Journal of Antimicrobial Chemotherapy 2006 58(2):310-314; doi:10.1093/jac/dkl223
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Antimicrobial resistance in non-typhoidal Salmonella from human sources, Spain, 2001–2003
1 Centro Nacional de Epidemiología, Instituto de Salud Carlos III Sinesio Delgado, 6, Madrid 28029, Spain 2 Centro Nacional de Microbiología, Instituto de Salud Carlos III, Carretera de Majadahonda a Pozuelo, s/n 28220 Majadahonda, Madrid, Spain 3 Agencia Española del Medicamento, Ministerio de Sanidad y Consumo, Carretera de Majadahonda a Pozuelo, s/n 28220 Majadahonda, Madrid, Spain
Received 5 December 2005; returned 14 February 2006; revised 28 March 2006; accepted 8 May 2006
*Corresponding author. Tel: +34-91-8222645; Fax: +34-91-3877815; E-mail: psoler{at}isciii.es
Objectives: To determine the current state of antimicrobial resistance among non-typhoidal Salmonella strains isolated from humans in Spain.
Methods: All strains of Salmonella from human sources received in the reference laboratory from 2001 to 2003 were serotyped and phage types were determined in the most common serovars. A systematic sampling procedure was carried out in order to obtain a random sample for susceptibility testing. The selected strains were tested for susceptibility to 12 different antimicrobial agents by a disc diffusion method using Mueller–Hinton agar. Results were scored as susceptible, moderately susceptible or resistant, according to CLSI criteria.
Results: From 2001 to 2003, 5777 strains of Salmonella were tested for susceptibility. Fifty per cent of strains of Salmonella Enteritidis were resistant to nalidixic acid. This was the most frequent resistance pattern of this serovar and it was characteristic of PT1, the most frequent phage type of Salmonella Enteritidis in Spain. Seventy-four per cent of Salmonella Typhimurium strains were resistant to four antibiotics or more. Resistance to ampicillin, chloramphenicol, streptomycin, sulphonamide and tetracycline was the most frequent resistance pattern of Salmonella Typhimurium and it was characteristic of DT104, the most frequent phage type in Spain. Sixty-nine per cent of Salmonella Hadar strains were resistant to at least four antibiotics.
Conclusions: The results of our study showed both a worrying percentage of strains of Salmonella Enteritidis resistant to nalidixic acid and of strains of Salmonella Typhimurium with a pattern of resistance to four antibiotics or more. Surveillance of antimicrobial resistance should carry on and improve in order to be able to evaluate the control measures carried out for decreasing resistance in Salmonella, specifically that addressed to the prudent use of antimicrobial agents by farmers and veterinarians.
Keywords: salmonellosis , zoonoses , drug-resistant
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