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JAC Advance Access originally published online on May 30, 2006
Journal of Antimicrobial Chemotherapy 2006 58(1):23-30; doi:10.1093/jac/dkl208
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© The Author 2006. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Assessment of allelic variation in the ccrAB locus in methicillin-resistant Staphylococcus aureus clones

Duarte C. Oliveira1,*, Catarina Milheiriço1, Susana Vinga2,{dagger} and Hermínia de Lencastre1,3

1 Laboratory of Molecular Genetics, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa Oeiras, Portugal 2 Biomathematics Group, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa Oeiras, Portugal 3 Laboratory of Microbiology, The Rockefeller University New York, NY 10021, USA

Received 10 February 2006; returned 11 April 2006; revised 27 April 2006; accepted 28 April 2006

*Correspondence address. Laboratory of Molecular Genetics, Instituto de Tecnologia Química e Biológica, Rua da Quinta Grande, 6, 2780-156 Oeiras, Portugal. Tel: +351-21-446-9862; Fax: +351-21-442-8766; E-mail: dco{at}itqb.unl.pt

Objectives: To evaluate the allelic variability of ccrA and ccrB among methicillin-resistant Staphylococcus aureus (MRSA) strains belonging to different genetic lineages and carrying different SCCmec types.

Methods: Internal fragments from both genes were obtained by PCR amplification with pairs of degenerate primers and the nucleotide sequence was determined for both strands. Nucleotide sequences were aligned and the phylogenetic trees were reconstructed.

Results: The homology scores obtained confirmed the very close relationships among ccrAB alleles associated with each SCCmec type, which further validate this locus as a good target for SCCmec typing strategies. Using this strategy, particularly for the ccrB gene, SCCmec types with the same ccrAB allotype, as detected by PCR, could be discriminated and some strains with non-typeable ccrAB loci were resolved.

Conclusions: We propose that sequencing an internal fragment of ccrB is a strategy for SCCmec typing that may be easily incorporated into other sequence-based MRSA typing strategies, such as multi-locus sequence typing and spa typing.

Keywords: MRSA , ccrAB typing , SCCmec typing


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