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JAC Advance Access originally published online on April 26, 2006
Journal of Antimicrobial Chemotherapy 2006 58(1):183-185; doi:10.1093/jac/dkl150
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© The Author 2006. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

A multiplex PCR for ß-lactamase genes of Haemophilus influenzae and description of a new blaTEM promoter variant

Stephen G. Tristram* and Scott Nichols

School of Human Life Sciences, University of Tasmania Launceston, Tasmania 7250, Australia

Received 25 January 2006; returned 23 March 2006; revised 28 March 2006; accepted 30 March 2006

*Corresponding author. Tel: +61-3-63-243323; Fax: +61-3-63-243658; E-mail: Stephen.Tristram{at}utas.edu.au

Objectives: To establish a multiplex PCR to detect and differentiate the ß-lactamase genes of Haemophilus influenzae.

Methods: A collection of H. influenzae isolates with a range of different ß-lactamase genes were tested in parallel with individual PCRs and the multiplex assay developed in this study.

Results: The multiplex method was found to be superior to previous methods and was able to correctly detect and differentiate blaROB-1 and the variants of blaTEM present in the strains tested, including a previously unrecognized variant associated with a 54 bp insertion in the promoter region.

Conclusions: The multiplex PCR will be a useful tool for the surveillance of ß-lactamase genes in H. influenzae.

Keywords: H. influenzae , bla genes , TEM, ROB-1


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