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JAC Advance Access originally published online on February 14, 2006
Journal of Antimicrobial Chemotherapy 2006 57(4):750-752; doi:10.1093/jac/dkl019
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© The Author 2006. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Heterogeneity of metallo-ß-lactamases in clinical isolates of Chryseobacterium meningosepticum from Hangzhou, China

Gong-Xiang Chen*, Rong Zhang and Hong Wei Zhou

2nd Affiliated Hospital of Zhejiang University, Zhejiang University, 88 JieFang Rd, Hangzhou 310009, China

Received 15 November 2005; returned 20 November 2005; revised 11 January 2006; accepted 13 January 2006

* Corresponding author. Tel: +86-571-8778-4633; Fax: +86-571-8782-4695; E-mail: chengong218{at}163.com

Objectives: To determine the distribution and heterogeneity of metallo-ß-lactamases (MBLs) responsible for imipenem resistance in Chryseobacterium meningosepticum.

Methods: Clinical C. meningosepticum isolates (n = 170) were collected from hospitals in Hangzhou, China. Production of MBLs was investigated by determination of imipenem MICs, and by using both a three-dimensional test and a 2-mercaptopropionic acid inhibitory test. Genes encoding BlaB and GOB MBLs were amplified by PCR, sequenced and compared with genes in GenBank.

Results: More than 95% of the 170 isolates showed high (MIC > 16 mg/L) or intermediate resistance to imipenem, but only 94 isolates (55%) were shown phenotypically to produce MBLs (imipenem MIC range, 8–256 mg/L), with MBL genes detected in 93 of these. Among them, 83 isolates had blaB alleles and 65 isolates had blaGOB alleles; 38 isolates possessed one MBL gene and 55 isolates contained two genes. The major blaB alleles encoded BlaB-2, -3 and -11, while the major blaGOB alleles encoded GOB-2, -4, -8 and -10. MBLs or their genes were not detected in 76 (45%) isolates, including many that were highly resistant to imipenem.

Conclusions: High levels and rates of imipenem resistance in C. meningosepticum from Hangzhou often result from the presence of heterogeneous BlaB and/or GOB MBLs, although undefined carbapenem resistance mechanisms also exist. Susceptibility testing and screening for MBLs should be conducted in order to inform effective treatment for C. meningosepticum infections.

Keywords: imipenem , resistance , genotypes , phenotypes


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