Skip Navigation


JAC Advance Access originally published online on December 6, 2005
Journal of Antimicrobial Chemotherapy 2006 57(2):335-338; doi:10.1093/jac/dki432
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
57/2/335    most recent
dki432v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (1)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Shackelford, J. C. N.
Right arrow Articles by Maillard, J.-Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shackelford, J. C. N.
Right arrow Articles by Maillard, J.-Y.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Use of a new alginate film test to study the bactericidal efficacy of the high-level disinfectant ortho-phthalaldehyde

J. C. N. Shackelford1, G. W. Hanlon1 and J.-Y. Maillard2,*

1 School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton BN2 4GJ, UK; 2 Welsh School of Pharmacy, Cardiff University, Redwood Building, King Edward VII Avenue, Cardiff CF10 3XL, UK

Received 4 March 2005; returned 19 April 2005; revised 22 June 2005; accepted 1 November 2005

* Corresponding author. Tel: +44(0)2920879088; Fax: +44(0)2920874149; E-mail: maillardj{at}cardiff.ac.uk

Objectives: To evaluate the merit of a new alginate efficacy film test to determine the bactericidal activity of the high-level disinfectant ortho-phthalaldehyde (OPA).

Methods: The efficacy of OPA was investigated using a new sodium alginate surface film test against Mycobacterium chelonae NCIMB 1474 and Epping, and Pseudomonas aeruginosa NCIMB 10421 under different test conditions.

Results: OPA was highly bactericidal against P. aeruginosa but its mycobactericidal efficacy was seriously reduced and produced ≥5 log reductions only at a concentration of 0.5% (w/v) within 30–60 min without organic load.

Conclusions: The sodium alginate film efficacy was reproducible between repeats. Inactivation results depended upon the concentration of OPA, contact time, the presence of an organic load and the bacterial genera.

Keywords: disinfection , time-kill assay , mycobacteria , Pseudomonas aeruginosa


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.