Skip Navigation


JAC Advance Access originally published online on December 13, 2005
Journal of Antimicrobial Chemotherapy 2006 57(2):199-203; doi:10.1093/jac/dki453
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
57/2/199    most recent
dki453v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (5)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Gould, V. C.
Right arrow Articles by Avison, M. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gould, V. C.
Right arrow Articles by Avison, M. B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

ß-Lactam resistance and ß-lactamase expression in clinical Stenotrophomonas maltophilia isolates having defined phylogenetic relationships

Virginia C. Gould, Aki Okazaki and Matthew B. Avison*

Bristol Centre for Antimicrobial Research and Evaluation, Department of Cellular and Molecular Medicine, University of Bristol, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK

Received 22 June 2005; returned 2 November 2005; revised 11 November 2005; accepted 15 November 2005

* Corresponding author. Tel: +44-117-9287528; Fax: +44-117-9287896; E-mail: Matthewb.Avison{at}bris.ac.uk

Aims: To test the hypothesis that Stenotrophomonas maltophilia isolates from certain phylogenetic groups have predictable ß-lactamase expression and ß-lactam resistance profiles.

Methods: Isolates were grouped using sequences of the 16S rRNA gene and smeT–smeD intergenic region. ß-Lactamase activities in cell extracts were quantified spectrophotometrically and ß-lactam MICs were determined using agar dilution methodology and Etest as appropriate.

Results: A collection of 50 clinical S. maltophilia isolates from Europe and North, South and Central America were phylogenetically grouped. Group ‘A’ (22 out of 50) includes remarkably genetically homogeneous isolates; group ‘B’ (17 out of 50) includes isolates that are genetically heterogeneous and quite distinct from those of group A. Members of these two groups are, however, indistinguishable in terms of their ß-lactam resistance and ß-lactamase expression phenotypes. In contrast, isolates from group ‘C’, which are less common (8 out of 50), are considerably more susceptible to ß-lactams owing to reduced inducibility of ß-lactamase expression following ß-lactam challenge.

Conclusions: The majority of S. maltophilia clinical isolates behave similarly in terms of ß-lactamase expression and ß-lactam resistance properties, despite considerable phylogenetic variability.

Keywords: expression of resistance , bacterial diversity , in vitro susceptibility


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Antimicrob. Agents Chemother.Home page
A. Okazaki and M. B. Avison
Induction of L1 and L2 {beta}-Lactamase Production in Stenotrophomonas maltophilia Is Dependent on an AmpR-Type Regulator
Antimicrob. Agents Chemother., April 1, 2008; 52(4): 1525 - 1528.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
Y. Fouhy, K. Scanlon, K. Schouest, C. Spillane, L. Crossman, M. B. Avison, R. P. Ryan, and J. M. Dow
Diffusible Signal Factor-Dependent Cell-Cell Signaling and Virulence in the Nosocomial Pathogen Stenotrophomonas maltophilia
J. Bacteriol., July 1, 2007; 189(13): 4964 - 4968.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
A. Okazaki and M. B. Avison
Aph(3')-IIc, an Aminoglycoside Resistance Determinant from Stenotrophomonas maltophilia
Antimicrob. Agents Chemother., January 1, 2007; 51(1): 359 - 360.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
V. C. Gould and M. B. Avison
SmeDEF-mediated antimicrobial drug resistance in Stenotrophomonas maltophilia clinical isolates having defined phylogenetic relationships
J. Antimicrob. Chemother., June 1, 2006; 57(6): 1070 - 1076.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.