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JAC Advance Access originally published online on November 9, 2005
Journal of Antimicrobial Chemotherapy 2006 57(1):61-70; doi:10.1093/jac/dki401
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© The Author 2005. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Molecular characteristics of pbp1a and pbp2b in clinical Streptococcus pneumoniae isolates in Quebec, Canada

Dominic Granger1,2, Geneviève Boily-Larouche1, Pierre Turgeon2,3, Karl Weiss2,4 and Michel Roger1,2,*

1 Laboratoire d'Immunogénétique, Centre de Recherche du Centre Hospitalier de l'Université de Montréal (CHUM), Hôpital Notre-Dame du CHUM, 1560 rue Sherbrooke est, Montréal, Québec, Canada H2L 4M1; 2 Département de Microbiologie-Immunologie de l'Université de Montréal, Montréal, Québec, Canada; 3 Département de Microbiologie, Hôpital Saint-Luc du CHUM, Montréal, Québec, Canada; 4 Département de Microbiologie, Hôpital Maisonneuve-Rosemont, Montréal, Québec, Canada

Received 20 May 2005; returned 25 June 2005; revised 15 August 2005; accepted 5 October 2005

* Corresponding author. Tel: +1-514-890-8000 ext. 25802; Fax: +1-514-412-7512; E-mail: michel.roger{at}ssss.gouv.qc.ca

Objectives: To investigate the nature of the amino acid motifs found in penicillin-binding protein (PBP) 2b and PBP1a of penicillin-resistant Streptococcus pneumoniae isolates across Quebec (Canada), and to obtain preliminary information regarding the prevalence of these alterations.

Methods: DNA sequences of pbp2b (codons 210–675) and pbp1a (codons 310–682) transpeptidase domains were determined and compared in 48 clinical isolates comprising 17 penicillin-susceptible (PSSP), 19 penicillin-intermediate (PISP) and 12 penicillin-resistant (PRSP) pneumococci.

Results: The degree of diversity within PBP1a and PBP2b correlated with increased resistance to ß-lactam antibiotics. There were an average of 0.6 ± 0.4 and 2.9 ± 0.2 mutations in PSSP, 16.8 ± 1.4 and 36.3 ± 5.2 in PISP, and 18.7 ± 2.5 and 51.4 ± 1.3 in PRSP isolates compared with control penicillin-susceptible R6-PBP2b and R6-PBP1a sequences, respectively. At least seven PBP2b and six PBP1a distinct amino acid profiles were identified among intermediate or resistant strains isolated in Quebec. The pattern of distribution of the PBPs' altered amino acids differs from that of other countries, with pneumococci isolates from Quebec showing a unique genetic signature.

Conclusion: This study will serve as a basis for future monitoring of genetic changes associated with the emergence and spread of ß-lactam resistance in Quebec, Canada.

Keywords: penicillin-binding proteins , ß-lactams , pneumococci , serotypes , penicillin resistance , cefotaxime resistance


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