Skip Navigation


JAC Advance Access originally published online on November 16, 2005
Journal of Antimicrobial Chemotherapy 2006 57(1):14-23; doi:10.1093/jac/dki398
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
57/1/14    most recent
dki398v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (27)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Eckert, C.
Right arrow Articles by Arlet, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Eckert, C.
Right arrow Articles by Arlet, G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published by Oxford University Press 2005

DNA sequence analysis of the genetic environment of various blaCTX-M genes

C. Eckert1, V. Gautier1 and G. Arlet1,2,*

1 Laboratoire de Bactériologie, UPRES EA 2392, Faculté de Médecine Pierre et Marie Curie, Université Paris VI, 27 rue de Chaligny, 75012 Paris, France; 2 Service de Bactériologie-Hygiène, Hôpital Tenon, Assistance Publique-Hôpitaux de Paris, 4 rue de la Chine, 75970 Paris cedex 20, France

Received 7 July 2005; returned 4 September 2005; revised 19 September 2005; accepted 5 October 2005

* Corresponding author. Tel: +33-1-56-01-70-18; Fax: +33-1-56-01-61-08; E-mail: guillaume.arlet{at}tnn.aphp.fr

Objectives: Over a 3 year period (2000–2003) 21 Escherichia coli, 5 Klebsiella pneumoniae, 1 Serratia marcescens and 1 Proteus mirabilis producing CTX-M-type ß-lactamase were collected from five different hospitals in Paris, France. This study was conducted to analyse the genetic environment of these 28 blaCTX-M genes.

Methods: Antimicrobial susceptibility testing was performed by the disc diffusion method and MICs of various ß-lactams were determined by an agar dilution method. PCR was used to detect and sequence alleles encoding CTX-M, TEM, SHV and CMY enzymes. The genetic environment was analysed by amplification and direct sequencing using various set of PCR primers or cloning in pBK-CMV.

Results: Sequence analysis revealed that these isolates contained seven different blaCTX-M genes: blaCTX-M-1 (4 strains), blaCTX-M-2 (2 strains), blaCTX-M-3 (4 strains), blaCTX-M-9 (1 strain), blaCTX-M-14 (5 strains), blaCTX-M-15 (11 strains), blaCTX-M-24 (1 strain). TEM-1 was associated with CTX-M-type enzymes in 15 isolates. Two strains produced both CTX-M-15 and SHV-2 or CTX-M-14 and CMY-2. In 25 strains the insertion sequence ISEcp1 was located upstream of the 5' end of the blaCTX-M gene. Among these strains, in five isolates, ISEcp1 was disrupted by insertion sequences such as IS26 (in three of them) or IS1 or IS10. Insertion sequence IS903 was found downstream of blaCTX-M-14 or blaCTX-M-24. Examination of the other three blaCTX-M genes (two blaCTX-M-2 and one blaCTX-M-9) by cloning, sequencing and PCR analysis revealed the presence of complex Class 1 integrons, In35, an integron similar to In60 and a novel integron.

Conclusions: This work further confirmed the predominant role of ISEcp1 in the mobilization of blaCTX-M genes of the CTX-M-1 cluster and the presence of In35, of an integron similar to In60 and a novel complex Class 1 integron.

Keywords: ESBLs , CTX-M ß-lactamases , insertion sequences , integrons


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Antimicrob. Agents Chemother.Home page
L. B. Rice, L. L. Carias, R. A. Hutton, S. D. Rudin, A. Endimiani, and R. A. Bonomo
The KQ Element, a Complex Genetic Region Conferring Transferable Resistance to Carbapenems, Aminoglycosides, and Fluoroquinolones in Klebsiella pneumoniae
Antimicrob. Agents Chemother., September 1, 2008; 52(9): 3427 - 3429.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
C. Lascols, I. Podglajen, C. Verdet, V. Gautier, L. Gutmann, C.-J. Soussy, E. Collatz, and E. Cambau
A Plasmid-Borne Shewanella algae Gene, qnrA3, and Its Possible Transfer In Vivo between Kluyvera ascorbata and Klebsiella pneumoniae
J. Bacteriol., August 1, 2008; 190(15): 5217 - 5223.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
Z. Su, X. Dai, J. Chen, F. Kong, H. Wang, Y. Li, S. Peng, S. Wang, Q. Shao, L. Lv, et al.
The blaCTX-M-1 gene located in a novel complex class I integron bearing an ISCR1 element in Escherichia coli isolates from Zhenjiang, China
J. Antimicrob. Chemother., July 21, 2008; (2008) dkn300v1.
[Full Text] [PDF]

Home page
 J Med MicrobiolHome page
L. Vinue, M. Lantero, Y. Saenz, S. Somalo, I. de Diego, F. Perez, F. Ruiz-Larrea, M. Zarazaga, and C. Torres
Characterization of extended-spectrum {beta}-lactamases and integrons in Escherichia coli isolates in a Spanish hospital
J. Med. Microbiol., July 1, 2008; 57(7): 916 - 920.
[Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
B. Haldorsen, B. Aasnaes, K. H. Dahl, A.-M. Hanssen, G. S. Simonsen, T. R. Walsh, A. Sundsfjord, and E. W. Lundblad
The AmpC phenotype in Norwegian clinical isolates of Escherichia coli is associated with an acquired ISEcp1-like ampC element or hyperproduction of the endogenous AmpC
J. Antimicrob. Chemother., June 26, 2008; (2008) dkn257v1.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
H. Mammeri, F. Eb, A. Berkani, and P. Nordmann
Molecular characterization of AmpC-producing Escherichia coli clinical isolates recovered in a French hospital
J. Antimicrob. Chemother., March 1, 2008; 61(3): 498 - 503.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
Z. Zong, S. R. Partridge, and J. R. Iredell
RmtC 16S rRNA Methyltransferase in Australia
Antimicrob. Agents Chemother., February 1, 2008; 52(2): 794 - 795.
[Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
A. Jouini, L. Vinue, K. B. Slama, Y. Saenz, N. Klibi, S. Hammami, A. Boudabous, and C. Torres
Characterization of CTX-M and SHV extended-spectrum {beta}-lactamases and associated resistance genes in Escherichia coli strains of food samples in Tunisia
J. Antimicrob. Chemother., November 1, 2007; 60(5): 1137 - 1141.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
A. G. Fernandez, A. Cloeckaert, A. Bertini, K. Praud, B. Doublet, F.-X. Weill, and A. Carattoli
Comparative Analysis of IncHI2 Plasmids Carrying blaCTX-M-2 or blaCTX-M-9 from Escherichia coli and Salmonella enterica Strains Isolated from Poultry and Humans
Antimicrob. Agents Chemother., November 1, 2007; 51(11): 4177 - 4180.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
C. Arpin, L. Coulange, V. Dubois, C. Andre, I. Fischer, S. Fourmaux, F. Grobost, J. Jullin, B. Dutilh, J.-F. Couture, et al.
Extended-Spectrum-{beta}-Lactamase-Producing Enterobacteriaceae Strains in Various Types of Private Health Care Centers
Antimicrob. Agents Chemother., September 1, 2007; 51(9): 3440 - 3444.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
I. K. Bae, Y.-N. Lee, W. G. Lee, S. H. Lee, and S. H. Jeong
Novel Complex Class 1 Integron Bearing an ISCR1 Element in an Escherichia coli Isolate Carrying the blaCTX-M-14 Gene
Antimicrob. Agents Chemother., August 1, 2007; 51(8): 3017 - 3019.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
L. Pallecchi, A. Bartoloni, C. Fiorelli, A. Mantella, T. Di Maggio, H. Gamboa, E. Gotuzzo, G. Kronvall, F. Paradisi, and G. M. Rossolini
Rapid Dissemination and Diversity of CTX-M Extended-Spectrum {beta}-Lactamase Genes in Commensal Escherichia coli Isolates from Healthy Children from Low-Resource Settings in Latin America
Antimicrob. Agents Chemother., August 1, 2007; 51(8): 2720 - 2725.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
D. Girlich, L. Poirel, A. Carattoli, I. Kempf, M.-F. Lartigue, A. Bertini, and P. Nordmann
Extended-Spectrum {beta}-Lactamase CTX-M-1 in Escherichia coli Isolates from Healthy Poultry in France
Appl. Envir. Microbiol., July 15, 2007; 73(14): 4681 - 4685.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
N. Mendonca, J. Leitao, V. Manageiro, E. Ferreira, the Antimicrobial Resistance Surveillance Program, and M. Canica
Spread of Extended-Spectrum {beta}-Lactamase CTX-M-Producing Escherichia coli Clinical Isolates in Community and Nosocomial Environments in Portugal
Antimicrob. Agents Chemother., June 1, 2007; 51(6): 1946 - 1955.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
A. Fernandez, E. Gil, M. Cartelle, A. Perez, A. Beceiro, S. Mallo, M. M. Tomas, F. J. Perez-Llarena, R. Villanueva, and G. Bou
Interspecies spread of CTX-M-32 extended-spectrum {beta}-lactamase and the role of the insertion sequence IS1 in down-regulating blaCTX-M gene expression
J. Antimicrob. Chemother., May 1, 2007; 59(5): 841 - 847.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
F. Depardieu, I. Podglajen, R. Leclercq, E. Collatz, and P. Courvalin
Modes and Modulations of Antibiotic Resistance Gene Expression
Clin. Microbiol. Rev., January 1, 2007; 20(1): 79 - 114.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
D. Costa, P. Poeta, Y. Saenz, L. Vinue, B. Rojo-Bezares, A. Jouini, M. Zarazaga, J. Rodrigues, and C. Torres
Detection of Escherichia coli harbouring extended-spectrum {beta}-lactamases of the CTX-M, TEM and SHV classes in faecal samples of wild animals in Portugal
J. Antimicrob. Chemother., December 1, 2006; 58(6): 1311 - 1312.
[Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
V. M. Ensor, M. Shahid, J. T. Evans, and P. M. Hawkey
Occurrence, prevalence and genetic environment of CTX-M {beta}-lactamases in Enterobacteriaceae from Indian hospitals
J. Antimicrob. Chemother., December 1, 2006; 58(6): 1260 - 1263.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
S. Ktari, G. Arlet, B. Mnif, V. Gautier, F. Mahjoubi, M. Ben Jmeaa, M. Bouaziz, and A. Hammami
Emergence of Multidrug-Resistant Klebsiella pneumoniae Isolates Producing VIM-4 Metallo-{beta}-Lactamase, CTX-M-15 Extended-Spectrum {beta}-Lactamase, and CMY-4 AmpC {beta}-Lactamase in a Tunisian University Hospital
Antimicrob. Agents Chemother., December 1, 2006; 50(12): 4198 - 4201.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
N. al Naiemi, B. Duim, and A. Bart
A CTX-M extended-spectrum {beta}-lactamase in Pseudomonas aeruginosa and Stenotrophomonas maltophilia.
J. Med. Microbiol., November 1, 2006; 55(Pt 11): 1607 - 1608.
[Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
F. Garnier, N. Raked, A. Gassama, F. Denis, and M.-C. Ploy
Genetic Environment of Quinolone Resistance Gene qnrB2 in a Complex sul1-Type Integron in the Newly Described Salmonella enterica Serovar Keurmassar.
Antimicrob. Agents Chemother., September 1, 2006; 50(9): 3200 - 3202.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
M. Lavollay, K. Mamlouk, T. Frank, A. Akpabie, B. Burghoffer, S. Ben Redjeb, R. Bercion, V. Gautier, and G. Arlet
Clonal Dissemination of a CTX-M-15 {beta}-Lactamase-Producing Escherichia coli Strain in the Paris Area, Tunis, and Bangui.
Antimicrob. Agents Chemother., July 1, 2006; 50(7): 2433 - 2438.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
A. J. C. Soler Bistue, F. A. Martin, A. Petroni, D. Faccone, M. Galas, M. E. Tolmasky, and A. Zorreguieta
Vibrio cholerae InV117, a Class 1 Integron Harboring aac(6')-Ib and blaCTX-M-2, Is Linked to Transposition Genes.
Antimicrob. Agents Chemother., May 1, 2006; 50(5): 1903 - 1907.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.