Skip Navigation


JAC Advance Access originally published online on November 30, 2005
Journal of Antimicrobial Chemotherapy 2006 57(1):110-115; doi:10.1093/jac/dki420
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
57/1/110    most recent
dki420v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (9)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Musset, L.
Right arrow Articles by Le Bras, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Musset, L.
Right arrow Articles by Le Bras, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Apparent absence of atovaquone/proguanil resistance in 477 Plasmodium falciparum isolates from untreated French travellers

Lise Musset1,2, Bruno Pradines3,4, Daniel Parzy4,5, Rémy Durand1,2, Patricia Bigot4,5 and Jacques Le Bras1,2,*

1 Centre National de Référence pour la Chimiosensibilité du Paludisme, APHP, Hôpital Bichat-Claude Bernard, Paris, France; 2 Laboratoire de Biologie Animale et Parasitaire, EA 209, Université Paris Descartes, Paris, France; 3 Unité de Recherche en Biologie et Epidémiologie Parasitaires, Institut de Médecine Tropicale du Service de Santé des Armées, Parc du Pharo, Marseille, France; 4 Institut Fédératif de Recherche 48, Marseille, France; 5 Unité de Recherche en Pharmacogénétique Parasitaire, Institut Médecine Tropicale du Service de Santé des Armées, Parc du Pharo, Marseille, France

Received 5 March 2005; returned 7 July 2005; revised 22 July 2005; accepted 24 October 2005

* Correspondence address. Laboratoire de Parasitologie, Hôpital Bichat-Claude Bernard, 46 Rue Henri Huchard, 75877 Paris cedex 18, France. Tel: +33-140-257-899; Fax: +33-140-256-763; E-mail: jacques.lebras{at}bch.ap-hop-paris.fr

Objectives: We examined the atovaquone in vitro susceptibility and the cytochrome b (cytb) gene polymorphism of African Plasmodium falciparum isolates during the first years of atovaquone/proguanil use.

Patients and methods: Between 1999 and 2004, we collected blood samples from French P. falciparum-infected patients returning from African countries. Atovaquone susceptibility was determined using an in vitro isotopic test and cytb genotyping was performed by restriction fragment length polymorphism analysis and sequencing. These results were analysed according to the clinical response to atovaquone/proguanil treatment.

Results: No in vitro atovaquone resistance (IC50 > 1900 nM) and no cytb mutation leading to the Y268S substitution were detected among 477 unexposed African P. falciparum isolates. Eight cytb polymorphisms were found outside the ubiquinone reduction site by sequencing the entire gene of 270 isolates. One atovaquone/proguanil treatment failure was documented; the post-treatment isolate had an atovaquone susceptibility of 8230 nM and the Ser268 Cytb change; the pre-treatment isolate, obtained 4 weeks previously, was Cytb Tyr268 (wild-type).

Conclusions: No atovaquone/proguanil resistance was detected by phenotyping or genotyping among 477 unexposed African P. falciparum isolates. Atovaquone/proguanil-resistant parasite was detectable only in the post-treatment isolate from a treatment failure.

Keywords: susceptibility , malaria , antimalarials , P. falciparum


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Antimicrob. Agents Chemother.Home page
H. Savini, H. Bogreau, L. Bertaux, H. Bouchiba, P. Kraemer, D. Parzy, E. Garnotel, C. Rogier, F. Simon, and B. Pradines
First Case of Emergence of Atovaquone-Proguanil Resistance in Plasmodium falciparum during Treatment in a Traveler in Comoros
Antimicrob. Agents Chemother., June 1, 2008; 52(6): 2283 - 2284.
[Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
P. Parola, B. Pradines, F. Simon, M.-P. Carlotti, P. Minodier, M.-P. Ranjeva, S. Badiaga, L. Bertaux, J. Delmont, M. Morillon, et al.
Antimalarial Drug Susceptibility and Point Mutations Associated with Drug Resistance in 248 Plasmodium falciparum Isolates Imported from Comoros to Marseille, France in 2004 2006
Am J Trop Med Hyg, September 1, 2007; 77(3): 431 - 437.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
E. Legrand, M. Demar, B. Volney, M.-T. Ekala, M. Quinternet, C. Bouchier, T. Fandeur, C. Rogier, B. Carme, O. M. Puijalon, et al.
First Case of Emergence of Atovaquone Resistance in Plasmodium falciparum during Second-Line Atovaquone-Proguanil Treatment in South America
Antimicrob. Agents Chemother., June 1, 2007; 51(6): 2280 - 2281.
[Full Text] [PDF]

Home page
 Am J Trop Med HygHome page
S. KRUDSOOD, S. N. PATEL, N. TANGPUKDEE, W. THANACHARTWET, W. LEOWATTANA, K. PORNPININWORAKIJ, A. K. BOGGILD, S. LOOAREESUWAN, and K. C. KAIN
EFFICACY OF ATOVAQUONE-PROGUANIL FOR TREATMENT OF ACUTE MULTIDRUG-RESISTANT PLASMODIUM FALCIPARUM MALARIA IN THAILAND
Am J Trop Med Hyg, April 1, 2007; 76(4): 655 - 658.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
D. J. Conway
Molecular Epidemiology of Malaria
Clin. Microbiol. Rev., January 1, 2007; 20(1): 188 - 204.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
B. Pradines, P. Hovette, T. Fusai, H. L. Atanda, E. Baret, P. Cheval, J. Mosnier, A. Callec, J. Cren, R. Amalvict, et al.
Prevalence of In Vitro Resistance to Eleven Standard or New Antimalarial Drugs among Plasmodium falciparum Isolates from Pointe-Noire, Republic of the Congo.
J. Clin. Microbiol., July 1, 2006; 44(7): 2404 - 2408.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.