Skip Navigation


JAC Advance Access originally published online on September 12, 2005
Journal of Antimicrobial Chemotherapy 2005 56(5):944-947; doi:10.1093/jac/dki289
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
56/5/944    most recent
dki289v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (7)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Wootton, M.
Right arrow Articles by Walsh, T. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wootton, M.
Right arrow Articles by Walsh, T. R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Published by Oxford University Press 2005

Reduced expression of the atl autolysin gene and susceptibility to autolysis in clinical heterogeneous glycopeptide-intermediate Staphylococcus aureus (hGISA) and GISA strains

Mandy Wootton1,2,*, Peter M. Bennett1, Alasdair P. MacGowan2 and Timothy R. Walsh1

Bristol Centre for Antimicrobial Research and Evaluation, 1 Department of Cellular and Molecular Medicine, Medical Sciences, University of Bristol, Bristol BS1 8TD, UK; and 2 North Bristol Healthcare Trust, Southmead Hospital, Bristol BS10 5NB, UK

Received 24 May 2005; returned 3 June 2005; revised 25 July 2005; accepted 26 July 2005

* Corresponding author. Tel: +44-117-928-7526; Fax: +44-117-928-7896; E-mail: mandy.wootton{at}bristol.ac.uk

Objectives: To assess a link between resistance to Triton X-100 induced autolysis (TIA) and lowered atl expression in a collection of clinical glycopeptide-intermediate Staphylococcus aureus (GISA) and heterogeneous GISA (hGISA).

Methods: Nine clinical GISA, 11 hGISA and 11 glycopeptide-susceptible S. aureus (GSSA), including three pairs of related isolates, were analysed using TIA assays. Lysostaphin MICs were determined by a broth microdilution technique and reverse transcriptase PCR was used to compare atl expression levels in all isolates.

Results: Eight of nine clinical GISA and six of 11 hGISA exhibited lower susceptibility to TIA and higher MICs of lysostaphin than GSSA. Eight of nine GISA and all hGISA strains had lowered atl expression levels compared with GSSA.

Conclusions: The majority of GISA and hGISA isolates exhibited lowered susceptibility to TIA and lysostaphin and reduced atl expression when compared with GSSA isolates. These factors could contribute to, or predispose to the development of, a thickened cell wall and glycopeptide-intermediate resistance.

Keywords: antibiotic resistance , cell wall , mechanism of resistance


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Clin. Microbiol.Home page
A. Yusof, A. Engelhardt, A. Karlsson, L. Bylund, P. Vidh, K. Mills, M. Wootton, and T. R. Walsh
Evaluation of a New Etest Vancomycin-Teicoplanin Strip for Detection of Glycopeptide-Intermediate Staphylococcus aureus (GISA), in Particular, Heterogeneous GISA
J. Clin. Microbiol., September 1, 2008; 46(9): 3042 - 3047.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
P. D. Stapleton, S. Shah, K. Ehlert, Y. Hara, and P. W. Taylor
The beta-lactam-resistance modifier (-)-epicatechin gallate alters the architecture of the cell wall of Staphylococcus aureus
Microbiology, July 1, 2007; 153(7): 2093 - 2103.
[Abstract] [Full Text] [PDF]

Home page
 J Med MicrobiolHome page
Z. Qin, X. Yang, L. Yang, J. Jiang, Y. Ou, S. Molin, and D. Qu
Formation and properties of in vitro biofilms of ica-negative Staphylococcus epidermidis clinical isolates
J. Med. Microbiol., January 1, 2007; 56(1): 83 - 93.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
A. Luczak-Kadlubowska, J. Krzyszton-Russjan, and W. Hryniewicz
Characteristics of Staphylococcus aureus Strains Isolated in Poland in 1996 to 2004 That Were Deficient in Species-Specific Proteins
J. Clin. Microbiol., November 1, 2006; 44(11): 4018 - 4024.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
A. Renzoni, C. Barras, P. Francois, Y. Charbonnier, E. Huggler, C. Garzoni, W. L. Kelley, P. Majcherczyk, J. Schrenzel, D. P. Lew, et al.
Transcriptomic and Functional Analysis of an Autolysis-Deficient, Teicoplanin-Resistant Derivative of Methicillin-Resistant Staphylococcus aureus.
Antimicrob. Agents Chemother., September 1, 2006; 50(9): 3048 - 3061.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
N. McCallum, H. Karauzum, R. Getzmann, M. Bischoff, P. Majcherczyk, B. Berger-Bachi, and R. Landmann
In Vivo Survival of Teicoplanin-Resistant Staphylococcus aureus and Fitness Cost of Teicoplanin Resistance.
Antimicrob. Agents Chemother., July 1, 2006; 50(7): 2352 - 2360.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
S. Utaida, R. F. Pfeltz, R. K. Jayaswal, and B. J. Wilkinson
Autolytic Properties of Glycopeptide-Intermediate Staphylococcus aureus Mu50.
Antimicrob. Agents Chemother., April 1, 2006; 50(4): 1541 - 1545.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.