JAC Advance Access originally published online on September 27, 2005
Journal of Antimicrobial Chemotherapy 2005 56(5):819-826; doi:10.1093/jac/dki345
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Characterization of the highly variable region surrounding the blaCTX-M-9 gene in non-related Escherichia coli from Barcelona
1 Servei de Microbiologia, Hospital de la Santa Creu i Sant Pau, 08025 Barcelona, Spain; 2 Unitat de Microbiologia, Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain; 3 Centre de Recerca en Sanitat Animal (CReSA), Universitat Autònoma de Barcelona-Institut de Recerca i Tecnologia Agroalimentària, 08193 Bellaterra, Spain
Received 24 March 2005; returned 30 June 2005; revised 18 July 2005; accepted 30 August 2005
* Correspondence address. Departament de Microbiologia, Hospital de la Santa Creu i Sant Pau, Avda Sant Antoni Ma Claret, 167, 08025 Barcelona, Spain. Tel: +34-93-291-9071; Fax: +34-93-291-9070; E-mail: fnavarror{at}santpau.es
Objectives: The dispersion of a clone, a plasmid or a mobile element carrying the blaCTX-M-9 gene was evaluated in 30 Escherichia coli strains isolated in Barcelona between 1996 and 1999. The presence of the previously described orf513-bearing class 1 integron, In60, carrying the blaCTX-M-9 gene, was also studied.
Methods: The clonality was analysed by pulsed-field gel electrophoresis. Plasmid analysis was performed by S1 digestion and hybridization with the CTX-M-9 probe. PCR mapping using specific designed primers was used to study the presence of In60 and In60-like structures.
Results: The clonality between the 30 strains was minor. The size of blaCTX-M-9 carrying plasmids ranged between 
80 and 430 kb. One strain produced only a chromosome-encoded CTX-M-9 ß-lactamase. Thirty-six per cent of the strains showed differences with respect to the In60 structure due to an insertion or deletion events.
Conclusions: These findings suggest that the bla CTX-M-9 gene may be carried by a mobile element that disperses it between plasmids. The fast dispersion of the CTX-M-9 enzyme could therefore be due to both diffusion of plasmids and mobile elements.
Keywords: ß-lactamases , plasmids , integrons
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