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JAC Advance Access originally published online on August 26, 2005
Journal of Antimicrobial Chemotherapy 2005 56(4):624-632; doi:10.1093/jac/dki306
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© The Author 2005. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org

Staphylococcal cassette chromosome mec (SCCmec) characterization and molecular analysis for methicillin-resistant Staphylococcus aureus and novel SCCmec subtype IVg isolated from bovine milk in Korea

Nam Hoon Kwon1, Kun Taek Park2, Jin San Moon3, Woo Kyung Jung1, So Hyun Kim1, Jun Man Kim1, Soon Keun Hong1, Hye Cheung Koo1, Yi Seok Joo3 and Yong Ho Park1,*

1 Department of Microbiology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Shillim dong, Gwanak gu, Seoul 151-742, Korea; 2 Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, WA, USA; 3 Department of Microbiology, National Veterinary Research and Quarantine Services, Anyang, Korea

Received 7 March 2005; returned 4 May 2005; revised 2 August 2005; accepted 4 August 2005

* Corresponding author. Tel: +82-2-880-1257; Fax: +82-2-871-7524; E-mail: yhp{at}snu.ac.kr

Objectives: To identify the staphylococcal cassette chromosome mec (SCCmec) types of methicillin-resistant Staphylococcus aureus (MRSA) isolated from bovine milk, and examine the genetic relatedness between MRSA from bovine milk and MRSA from human isolates.

Methods: Antimicrobial susceptibility tests were performed on MRSA isolated from bovine milk. PCR and sequencing analysis were performed to determine the SCCmec type of MRSA, and to confirm their toxin carriage. Genetic relatedness among the bovine isolates and between bovine and human isolates was detected with PFGE and multilocus sequence typing (MLST).

Results: Fourteen MRSA and a silent mecA-carrying methicillin-susceptible S. aureus (smMSSA) were isolated from the milk of cows with an isolation ratio of 0.18%. SCCmec of 14 MRSA strains were designated as new subtype IVg, and one smMSSA strain was not classified. All 14 MRSA strains shared Panton-Valentine leucocidin (PVL) and staphylococcal enterotoxin D (SED), SEI and SEJ; the smMSSA strain had only PVL. All MRSA and smMSSA isolates showed no multidrug resistance and had community-acquired MRSA (CA-MRSA) characteristics. PFGE revealed that all isolates except the smMSSA belonged to the same genetic lineage, and MLST analysis showed that they had no genetic relatedness with CA-MRSA which had caused human infection in Korea.

Conclusions: MRSA isolated from bovine milk harboured a unique SCCmec subtype, and they may not be correlated with the emergence of CA-MRSA in human infection in Korea.

Keywords: MRSA , PFGE , MLST


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