JAC Advance Access originally published online on July 20, 2005
Journal of Antimicrobial Chemotherapy 2005 56(3):586-589; doi:10.1093/jac/dki250
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Plasmid-mediated resistance to ciprofloxacin and cefotaxime in clinical isolates of Salmonella enterica serotype Enteritidis in Hong Kong
1 Microbiology Division, Public Health Laboratory Services Branch, Centre for Health Protection, Department of Health, Hong Kong SAR, China; 2 Infection Control Branch, Centre for Health Protection, Department of Health, Hong Kong SAR, China
Received 22 April 2005; returned 6 June 2005; revised 19 June 2005; accepted 20 June 2005
* Corresponding author. Tel: +852-2319-8685; Fax: +852-2776-1446; E-mail: alf{at}chp.gov.hk
Objectives: To characterize the genetic determinants involved in the reduced susceptibility to ciprofloxacin and cefotaxime in Salmonella enterica serotype Enteritidis clinical isolates obtained from four patients in summer 2003 in Hong Kong.
Methods: Three Salmonella Enteritidis isolates from blood culture and one from stool were collected due to their increased resistance to ciprofloxacin and cefotaxime. PFGE analysis was used to investigate their genetic relatedness. Conjugation experiments were employed to show if the genetic determinants involved were plasmid-mediated. MICs of various antimicrobials were determined by VITEK 2 and Etest. Based on the susceptibility and conjugation experiment results, previously described PCR methods were employed to detect sequences homologous to qnr and blaCTX-M suspected to be involved in the reduced susceptibility to ciprofloxacin and cefotaxime, respectively.
Results: PFGE analysis showed that the four Salmonella isolates were clonally unrelated. The presence of a qnr-like gene and the CTX-M allele blaCTX-M-14 on four different transferable plasmids harboured by the four isolates was confirmed.
Conclusions: This is the first report of transferable fluoroquinolone resistance due to a new qnr allele, which appeared to be linked to blaCTX-M-14, in isolates of Salmonella Enteritidis in Hong Kong.
Keywords: Qnr , CTX-M-14 , extended-spectrum ß-lactamases , ESBLs
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