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JAC Advance Access originally published online on July 8, 2005
Journal of Antimicrobial Chemotherapy 2005 56(3):481-484; doi:10.1093/jac/dki238
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© The Author 2005. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oupjournals.org

Variation in selected regions of blaTEM genes and promoters in Haemophilus influenzae

Stephen G. Tristram*, Rebecca Hawes and Juliana Souprounov

School of Human Life Sciences, University of Tasmania, Launceston, Tasmania 7250, Australia

Received 2 March 2005; returned 26 April 2005; revised 24 May 2005; accepted 8 June 2005

* Corresponding author. Tel: +61-3-63-245469; Fax: +61-3-63-243658; E-mail: Stephen.Tristram{at}utas.edu.au

Objectives: To determine if the TEM ß-lactamases of Haemophilus influenzae are TEM-1 or derivatives thereof and associated exclusively with the overlapping Pa/Pb promoters.

Methods: Single nucleotide specific PCR was used to discriminate the polymorphic nucleotides at positions 32 and 317 of the blaTEM genes of a collection of TEM-positive strains.

Results: All blaTEM genes were found to be blaTEM-1 or derivatives thereof and none blaTEM-2. The blaTEM genes were associated with the P3 promoter, the Pa/Pb promoters or a novel promoter produced as a result of a 135 bp deletion and a G162T substitution.

Conclusions: The genetic features of blaTEM genes in H. influenzae are different from those in Enterobacteriaceae and more variable than previously recognized.

Keywords: H. influenzae , promoters , ß-lactamases , blaTEM genes , TEM


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