JAC Advance Access originally published online on June 24, 2005
Journal of Antimicrobial Chemotherapy 2005 56(2):413-415; doi:10.1093/jac/dki228
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Colicins prevent colonization of urinary catheters
1 Department of Medicine, Infectious Diseases Section, Baylor College of Medicine, Houston, TX, USA; 2 Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, USA; 3 Department of Physical Medicine and Rehabilitation, Baylor College of Medicine, Houston, TX, USA; 4 Center for Prostheses Infection, Baylor College of Medicine, Houstor, TX, USA; 5 Michael E. DeBakey Veterans Affairs Medical Center, Houston, TX, USA
Received 16 March 2005; returned 19 May 2005; revised 27 May 2005; accepted 2 June 2005
* Correspondence address. Spinal Cord Injury (128), MEDVAMC, 2002 Holcombe Boulevard, Houston, TX 77030, USA. Tel: +1-713-791-1414-1-3251; Fax: +1-713-794-8005; E-mail: trautner{at}bcm.tmc.edu
Objectives: Natural microbial defence systems, such as bacteriocins, may be a novel means to prevent catheter-associated urinary tract infection. We investigated in vitro whether a colicin-expressing strain of Escherichia coli could prevent urinary catheter colonization by a colicin-susceptible, uropathgenic strain of E. coli.
Methods: Segments of urinary catheter were inoculated with colicin-producing E. coli K-12 and then exposed to either colicin-susceptible E. coli (a uropathogenic clinical isolate) or colicin-resistant E. coli (derived from the susceptible clinical isolate). Catheters were then incubated overnight, rinsed and sonicated.
Results: The presence of colicin-producing E. coli K-12 on the catheter surface completely prevented catheter colonization by colicin-susceptible E. coli but not by resistant E. coli. The colicin-susceptible strain but not the colicin-resistant strain also disappeared from broth cultures in the presence of colicin-producing E. coli K-12.
Conclusions: The observed inhibition of catheter colonization by the uropathogenic clinical isolate of E. coli can be attributed to the presence of a colicin-producing strain of E. coli on the catheter surface. Bacteriocin production by a non-pathogenic organism may have clinical applicability as a means to prevent catheter-associated urinary tract infection.
Keywords: bacteriocins , UTIs , uropathogens
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