Skip Navigation


JAC Advance Access originally published online on July 4, 2005
Journal of Antimicrobial Chemotherapy 2005 56(2):396-398; doi:10.1093/jac/dki242
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
56/2/396    most recent
dki242v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (1)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Rolain, J. M.
Right arrow Articles by Raoult, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rolain, J. M.
Right arrow Articles by Raoult, D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oupjournals.org

Prediction of resistance to erythromycin in the genus Rickettsia by mutations in L22 ribosomal protein

J. M. Rolain and D. Raoult*

Unité desxs Rickettsies, CNRS UMR 6020, IFR 48, Faculté de Médecine, Université de la Méditerranée, 27 Bd Jean Moulin, 13385 Marseille Cedex 05, France

Received 6 January 2005; returned 19 May 2005; revised 7 May 2005; accepted 15 June 2005

* Corresponding author. Tel: +33-4-91-38-55-17; Fax: +33-4-91-83-03-90; E-mail: Didier.Raoult{at}medecine.univ-mrs.fr

Objectives: Typhus group (TG) rickettsiae are naturally susceptible to erythromycin whereas spotted fever group (SFG) rickettsioses are not. The aim of this study was to compare in silico genetic determinants known to be associated with resistance to macrolide compounds.

Methods and results: Available sequences of the 23S RNA gene, and L4 and L22 ribosomal proteins of rickettsial strains were aligned and compared using in silico methods. Although there were no sequence differences in domain V of the 23S RNA gene and in the conserved region of the L4 ribosomal protein gene, we found that TG rickettsiae had a triple amino acid difference in the highly conserved region of the L22 ribosomal protein compared with the SFG rickettsiae.

Conclusions: We believe that the triple amino acid difference in the L22 ribosomal protein found in this study may explain the difference in susceptibility to erythromycin among the Rickettsia genus. Genome analysis may help to predict possible molecular mechanisms of resistance for fastidious and intracellular bacteria and cloning and expression of such proteins should be investigated in the future in order to prove our hypothesis.

Keywords: antibiotic resistance , genome analysis , in silico , L22 protein , erythromycin resistance , macrolides


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.