JAC Advance Access originally published online on May 25, 2005
Journal of Antimicrobial Chemotherapy 2005 56(1):133-138; doi:10.1093/jac/dki167
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Susceptibility of European ß-lactamase-positive and -negative Haemophilus influenzae isolates from the periods 1997/1998 and 2002/2003
Eijkman-Winkler Center, University Medical Center Utrecht, 3508 GA Utrecht, The Netherlands
Received 15 December 2004; returned 27 January 2005; revised 19 April 2005; accepted 19 April 2005
* Corresponding author. Tel: +31-30-2507630; Fax: +31-30-2541770; E-mail: A.C.Fluit{at}lab.azu.nl
Aims: To test prospectively the activity of cefixime and comparators against Haemophilus influenzae from Europe and to compare the susceptibilities of isolates from 1997/1998 with isolates from 2002/2003 paying special attention to the epidemiology of amoxicillin resistance.
Methods: MICs of antibiotics were determined using broth microdilution. For ß-lactamase-negative isolates with reduced susceptibility to amoxicillin, the nucleotide sequence of the penicillin-binding domain of PBP3 was determined.
Results: The prevalence of ß-lactamase-positive isolates in certain countries has reached 38%. During the period 1997/1998, 8.8% of the isolates were ß-lactamase-negative and non-susceptible to amoxicillin (BLNAR). During the period 2002/2003, 9.6% of the isolates were BLNAR. The emergence of the BLNAR phenotype of H. influenzae was demonstrated in all countries with a prevalence ranging from 2% to 20%. The penicillin-binding domain of PBP3 from 30 sequenced isolates showed known amino acid substitutions, although no amino acid changes were observed in two BLNAR isolates. Clonal spread of BLNAR strains was limited or absent in our study. Both ß-lactamase-producing and BLNAR strains of H. influenzae were fully susceptible to cefixime. However, neither ß-lactamase-producing nor BLNAR isolates were fully susceptible to the other cephalosporins tested. All isolates were also fully susceptible to levofloxacin, moxifloxacin, azithromycin and telithromycin.
Conclusions: The prevalence of the BLNAR phenotype in Europe is increasing, but no new amino acid substitutions were detected in the penicillin-binding domain of PBP3. Cefixime remains a useful treatment option for respiratory tract infections, including in areas with increasing resistance problems.
Keywords: PBP3 , surveillance , cefixime
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Garcia-Cobos, J. Campos, E. Cercenado, F. Roman, E. Lazaro, M. Perez-Vazquez, F. de Abajo, and J. Oteo Antibiotic Resistance in Haemophilus influenzae Decreased, except for {beta}-Lactamase-Negative Amoxicillin-Resistant Isolates, in Parallel with Community Antibiotic Consumption in Spain from 1997 to 2007 Antimicrob. Agents Chemother., August 1, 2008; 52(8): 2760 - 2766. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Garcia-Cobos, J. Campos, F. Roman, C. Carrera, M. Perez-Vazquez, B. Aracil, and J. Oteo Low {beta}-Lactamase-Negative Ampicillin-Resistant Haemophilus influenzae Strains Are Best Detected by Testing Amoxicillin Susceptibility by the Broth Microdilution Method Antimicrob. Agents Chemother., July 1, 2008; 52(7): 2407 - 2414. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Gracia, C. Diaz, P. Coronel, M. Gimeno, R. Garcia-Rodas, G. del Prado, L. Huelves, V. Ruiz, P. L. Naves, M. C. Ponte, et al. Antimicrobial susceptibility of Haemophilus influenzae and Moraxella catarrhalis isolates in eight Central, East and Baltic European countries in 2005-06: results of the Cefditoren Surveillance Study J. Antimicrob. Chemother., May 1, 2008; 61(5): 1180 - 1181. [Full Text] [PDF]
|
|
|
|
|
|
S. G. Tristram, M. J. Pitout, K. Forward, S. Campbell, S. Nichols, and R. J. Davidson Characterization of extended-spectrum {beta}-lactamase-producing isolates of Haemophilus parainfluenzae J. Antimicrob. Chemother., March 1, 2008; 61(3): 509 - 514. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hotomi, K. Fujihara, D. S. Billal, K. Suzuki, T. Nishimura, S. Baba, and N. Yamanaka Genetic Characteristics and Clonal Dissemination of {beta}-Lactamase-Negative Ampicillin-Resistant Haemophilus influenzae Strains Isolated from the Upper Respiratory Tract of Patients in Japan Antimicrob. Agents Chemother., November 1, 2007; 51(11): 3969 - 3976. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Garcia-Cobos, J. Campos, E. Lazaro, F. Roman, E. Cercenado, C. Garcia-Rey, M. Perez-Vazquez, J. Oteo, and F. de Abajo Ampicillin-Resistant Non-{beta}-Lactamase-Producing Haemophilus influenzae in Spain: Recent Emergence of Clonal Isolates with Increased Resistance to Cefotaxime and Cefixime Antimicrob. Agents Chemother., July 1, 2007; 51(7): 2564 - 2573. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Tristram, M. R. Jacobs, and P. C. Appelbaum Antimicrobial Resistance in Haemophilus influenzae Clin. Microbiol. Rev., April 1, 2007; 20(2): 368 - 389. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I.-S. Kim, C.-S. Ki, S. Kim, W. S. Oh, K. R. Peck, J.-H. Song, K. Lee, and N. Y. Lee Diversity of Ampicillin Resistance Genes and Antimicrobial Susceptibility Patterns in Haemophilus influenzae Strains Isolated in Korea Antimicrob. Agents Chemother., February 1, 2007; 51(2): 453 - 460. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. A. Pankuch, D. Hoellman, A. Bryskier, J. Lowther, and P. C. Appelbaum Effects of Various Media on the Activity of NXL103 (Formerly XRP 2868), a New Oral Streptogramin, against Haemophilus influenzae Antimicrob. Agents Chemother., November 1, 2006; 50(11): 3914 - 3916. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. T. M. Jansen, A. Verel, M. Beitsma, J. Verhoef, and D. Milatovic Longitudinal European surveillance study of antibiotic resistance of Haemophilus influenzae J. Antimicrob. Chemother., October 1, 2006; 58(4): 873 - 877. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Sanbongi, T. Suzuki, Y. Osaki, N. Senju, T. Ida, and K. Ubukata Molecular Evolution of {beta}-Lactam-Resistant Haemophilus influenzae: 9-Year Surveillance of Penicillin-Binding Protein 3 Mutations in Isolates from Japan. Antimicrob. Agents Chemother., July 1, 2006; 50(7): 2487 - 2492. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Bozdogan, S. Tristram, and P. C. Appelbaum Combination of altered PBPs and expression of cloned extended-spectrum {beta}-lactamases confers cefotaxime resistance in Haemophilus influenzae J. Antimicrob. Chemother., April 1, 2006; 57(4): 747 - 749. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Skoczynska, M. Kadlubowski, J. Empel, and W. Hryniewicz Characteristics of Haemophilus influenzae Type b Responsible for Meningitis in Poland from 1997 to 2004 J. Clin. Microbiol., November 1, 2005; 43(11): 5665 - 5669. [Abstract] [Full Text] [PDF]
|
|