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JAC Advance Access originally published online on May 12, 2005
Journal of Antimicrobial Chemotherapy 2005 56(1):122-127; doi:10.1093/jac/dki160
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© The Author 2005. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oupjournals.org

Prevalence of Ambler class A and D ß-lactamases among clinical isolates of Pseudomonas aeruginosa in Korea

Seungok Lee1, Yeon-Joon Park2,*, Myungshin Kim2, Hae Kyung Lee2, Kyungja Han2, Chang Suk Kang2 and Moon Won Kang3

1 Seoul Medical Science Institute, Seoul Clinical Laboratories, Seoul, Korea; 2 Department of Clinical Pathology, College of Medicine, The Catholic University of Korea, Kangnam St Mary's Hospital, 505 Banpo-dong, Seocho-ku, Seoul, 137-701, Korea; 3 Department of Internal Medicine, College of Medicine, The Catholic University of Korea, Seoul, Korea

Received 1 February 2005; returned 7 March 2005; revised 11 April 2005; accepted 15 April 2005

* Corresponding author. Tel: +82-2-590-1604; Fax: +82-2-592-4190; Email: yjpk{at}catholic.ac.kr

Objectives: Recently, resistance to extended-spectrum cephalosporins due to acquired ß-lactamases has been reported in Pseudomonas aeruginosa. The aim of this study was to investigate the prevalence of Ambler class A and D ß-lactamases and their extended-spectrum derivatives and antimicrobial susceptibilities of P. aeruginosa isolated from various parts of Korea.

Methods: A total of 252 consecutive, non-duplicate isolates of P. aeruginosa were studied for the presence of class A or D ß-lactamase. Antibiotic susceptibility tests and PCR amplification of genes encoding class A (blaPSE-1, blaPER-1, blaVEB-1, blaTEM, blaSHV, blaCTX-M and blaGES-1) and class D ß-lactamases (blaOXA-groupI, blaOXA-groupII and blaOXA-groupIII) were performed. For PCR-positive isolates, isoelectric focusing (IEF) analysis, sequencing and pulsed-field gel electrophoresis (PFGE) were performed.

Results: In 64 (25.4%) isolates, structural genes for PSE-1 (6.3%), OXA-10 (13.1%), OXA-4 (4.3%), OXA-30 (2.0%), OXA-2 (2.3%) and OXA-17 (0.4%) were found; their distribution varied between provinces. None harboured blaPER-1, blaVEB-1, blaTEM, blaSHV, blaCTX-M and blaGES-1. The cross-class resistance rates to other antibiotics was significantly higher in class A and D ß-lactamase producers than in non-producers (P < 0.001 for aminoglycosides, ciprofloxacin and meropenem).

Conclusions: OXA-type ß-lactamases are widespread, but their extended-spectrum derivatives are rare among P. aeruginosa in Korea. To our knowledge, this is the first report of OXA-17, an extended-spectrum derivative of OXA-10, outside the Middle East. In addition, combined resistance to ticarcillin and aminoglycosides was a useful indicator for P. aeruginosa producing PSE- or OXA-type ß-lactamases in this study.

Keywords: P. aeruginosa , ESBLs , extended-spectrum ß-lactamases , OXA-17


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