Skip Navigation


JAC Advance Access originally published online on April 28, 2005
Journal of Antimicrobial Chemotherapy 2005 55(6):879-882; doi:10.1093/jac/dki123
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
55/6/879    most recent
dki123v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (3)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Escalada, M. G.
Right arrow Articles by Ochs, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Escalada, M. G.
Right arrow Articles by Ochs, D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions{at}oupjournals.org

Triclosan inhibition of fatty acid synthesis and its effect on growth of Escherichia coli and Pseudomonas aeruginosa

Margarita Gomez Escalada1, J. L. Harwood2, J.-Y. Maillard1,* and D. Ochs3

1 Welsh School of Pharmacy, Cardiff University, Cardiff CF10 3XF; 2 Cardiff School of Biosciences, Cardiff University, Cardiff CF10 3TL, UK; 3 Ciba Specialty Chemicals Inc., PO Box 1266, D-79630 Grenzach-Wyhlen, Germany

* Corresponding author. Tel: +44-29-2087-9088; Fax: +44-29-2087-4149; Email: maillardj{at}cardiff.ac.uk

Objectives: To assess the effect of triclosan on fatty acid synthesis and to relate the inhibition of enoyl reductase to bacterial viability.

Methods: The effect of triclosan on fatty acid synthesis in a triclosan-resistant Escherichia coli and its sensitive counterpart and in Pseudomonas aeruginosa was investigated by measuring acetate incorporation into total lipid followed by analysis of fatty acid methyl esters by gas chromatography. Concurrently, the bactericidal effect of triclosan against these bacterial strains was assessed.

Results: Triclosan inhibited fatty acid biosynthesis in all the strains tested. However, for triclosan-resistant E. coli (MIC > 1000 mg/L) the concentration required to achieve inhibition was higher than that required for the susceptible counterpart. These concentrations did not significantly affect cell survival in any of the strains tested.

Conclusions: This study shows that the inhibition of fatty acid biosynthesis by the bisphenol might be involved in its growth-inhibitory action and that other mechanisms are involved in its lethal effect. In addition, although microorganisms with a high triclosan MIC were still susceptible to the inhibitory effect of the bisphenol on fatty acid biosynthesis, a higher concentration of the compound was required. This suggested that triclosan bioavailability was different in these strains.

Keywords: enoyl reductase , mechanism of action , bacteriostatic , bactericidal


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Ann. Thorac. Surg.Home page
T. Fleck, R. Moidl, A. Blacky, M. Fleck, E. Wolner, M. Grabenwoger, and W. Wisser
Triclosan-Coated Sutures for the Reduction of Sternal Wound Infections: Economic Considerations
Ann. Thorac. Surg., July 1, 2007; 84(1): 232 - 236.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.