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JAC Advance Access originally published online on March 16, 2005
Journal of Antimicrobial Chemotherapy 2005 55(5):680-685; doi:10.1093/jac/dki065
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© The Author 2005. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions{at}oupjournals.org

Genetic and biochemical characterization of the chromosome-encoded class B ß-lactamases from Shewanella livingstonensis (SLB-1) and Shewanella frigidimarina (SFB-1)

Laurent Poirel, Claire Héritier and Patrice Nordmann*

Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris,
Faculté de Médecine Paris-Sud, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre Cedex, France

* Corresponding author. Tel: +33-1-45-21-36–32; Fax: +33-1-45-21-63-40; Email: nordmann.patrice{at}bct.ap-hop-paris.fr

Objectives: To determine the ß-lactamase gene content of ß-lactam-susceptible psychrophilic Gram-negative bacilli, Shewanella frigidimarina and Shewanella livingstonensis, isolated from a marine environment.

Methods: ß-Lactamase genes were cloned, sequenced and expressed in Escherichia coli. Kinetic parameters were determined using purified enzymes.

Results: Metallo-ß-lactamases SLB-1 and SFB-1 were identified from S. livingstonensis and S. frigidimarina, respectively, sharing 65% amino acid identity and being distantly related to other Ambler class B ß-lactamases (40 and 36% amino acid identity with GIM-1 and IMP-1 from Pseudomonas aeruginosa, respectively). SLB-1 had an EDTA-inhibited and broad-spectrum ß-lactam hydrolysis profile, whereas SFB-1 did not hydrolyse cephalosporins, with activity being weakly inhibited by EDTA and dipicolinic acid.

Conclusions: This work provides further evidence that psychrophilic bacterial species may constitute a reservoir of ß-lactam resistance genes.

Keywords: metallo-ß-lactamases , S. livingstonensis , S. frigidimarina , carbapenems


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